| 基因治疗帕金森病的实验研究 |
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| 引用本文： | 唐镇生,宋后燕.基因治疗帕金森病的实验研究[J].中国临床神经科学,1995,3(4):194-196. |
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| 作者姓名： | 唐镇生 宋后燕 |
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| 作者单位： | 上海医科大学神经病学研究所 200040
(唐镇生，应其龙，江澄川)，上海医科大学分子遗传研究室 200032
(宋后燕)，上海医科大学分子遗传研究室 200032(崔宁) |
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| 基金项目： | 国家自然科学基金(编号39470713) |
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| 摘 要： | 本研究把Ⅰ型人酪氨酸羟化酶(human tyrosine hydroxylase type Ⅰ;HTH_1)cDNA连接到逆转录病毒载体LNSX上,构建成真核表达载体 LNSHTH_1,然后通过in vivo途径转染帕金森病模型鼠的纹状体细胞。外源的HTH_1基因在宿主脑内得到了表达,并使其病理行为改善约70％。这种新的基因治疗帕金森病的方式,有其独特的优越性。
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| 关 键 词： | 帕金森病 基因治疗 基因转移 |
Experimental Study of Intracerebral Transplantation of Recombinant Gene for Parkinson' s Disease |
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| Tang Zhen-Sheng,Ying Qi-Long,Jiang Cheng-Chuan,Song Hou-Yan,Cui Ning
Institute of Neurology.Experimental Study of Intracerebral Transplantation of Recombinant Gene for Parkinson'''' s Disease[J].Chinese Journal of Clinical Neurosciences,1995,3(4):194-196. |
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| Authors: | Tang Zhen-Sheng Ying Qi-Long Jiang Cheng-Chuan Song Hou-Yan Cui Ning
Institute of Neurology |
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| Institution: | Tang Zhen-Sheng,Ying Qi-Long,Jiang Cheng-Chuan,Song Hou-Yan,Cui Ning
Institute of Neurology,Department of Molecular Genetics Shanghai Medical University 200040 |
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| Abstract: | We constructed an eukaryotic expression plasmid LNSHTH1 by inserting human tyrosin hydroxylase type I (HTH:) cDNA into the retroviral vector LNSX. The recombinant plas mid LNSHTHi was transfected into the striatum of Parkinson's disease (PD)rats in vivo. E pression of the foreign HTH gene was found by TH immunohistochemistry and rotational be havior of the PD rats was amelirated by 70 per cent. This new method of gene therapy for P. offers some unique advantages. |
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| Keywords: | Parkinson's disease Gene therapy Gene transfer |
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