氯诺昔康联合地佐辛超前镇痛对切口痛大鼠白细胞介素-6及脊髓环氧化酶-2表达的影响

目的:观察氯诺昔康联合地佐辛对切口痛大鼠模型的超前镇痛效应,探讨其可能作用机制。方法:40只Wistar大鼠随机分为空白对照组、切口痛模型组、氯诺昔康组、地佐辛组和氯诺昔康联合地佐辛组(联合用药组)各8只。空白对照组不做任何处理;切口痛模型组术前30 min尾静脉注射0.9%氯化钠注射液;氯诺昔康组术前30 min尾静脉注射氯诺昔康2 mg/kg;地佐辛组术前30 min尾静脉注射地佐辛5 mg;联合用药组术前30 min尾静脉注射氯诺昔康1 mg/kg,地佐辛2.5 mg。术后采用累计疼痛评分法给予切口痛大鼠疼痛评分,术后3h大鼠尾静脉采血,于脊髓膨大处取脊髓,分别检测血清白细胞介素(IL)-6和脊髓环氧化酶(COX)-2的含量。结果:切口痛模型组大鼠累计疼痛评分均明显高于氯诺昔康组、地佐辛组和联合用药组(P<0.01),而联合用药组大鼠累计疼痛评分均较单用药组显著下降(P<0.01);联合用药组大鼠血清IL-6和脊髓COX-2的表达均显著低于单用药组(P<0.01)。结论:氯诺昔康联合地佐辛能通过抑制切口痛大鼠血清IL-6和脊髓COX-2的表达,降低大鼠累计疼痛评分,表现出显著的超前镇痛作用。

Effect of lornoxicam combined with dezocine preemptive analgesia on the expressions of serum interleukin-6 and cyclooxygenase-2 in spinal cord in rats with incision pain

Objective:To observe the preemptive analgesia effects of lornoxicam combined with dezocine on the rat with incision pain, and investigate its possible mechanism. Methods:Forty Wistar rats were randomly divided into the control group ( without any treatment),incision pain model group(treatment with preoperative 30 min tail vein injection of saline),lornoxicam group(treatment with preoperative 30 min tail vein injection of 2 mg/kg of lornoxicam),dezocine group(treatment with preoperative 30min tail vein injection of 5 mg of lornoxicam) and lornoxicam combined with dezocine group( treatment with preoperative 30 min tail vein injection of 1 mg/kg of lornoxicam combined with 2. 5 mg of lornoxicam). The pain score of rats with incision pain after operation was investigated using the cumulative pain score method. The interleukin(IL)-6 expression in the rat tail vein and cyclooxygenase(COX)-2 level in spinal cord were measured after 3h of operation. Results:The cumulative pain score in incision pain model group was significantly higher than that in lornoxicam group,dezocine group and lornoxicam combined with dezocine group(P<0. 01). The cumulative pain score in lornoxicam combined with dezocine group was significantly lower than that in single drug group(P<0. 01). The expressions of serum IL-6 and COX-2 in spinal cord in lornoxicam combined with dezocine group were significantly lower than that in single drug group (P<0. 01). Conclusions:Lornoxicam combined with dezocine can inhibit the expressions of serum IL-6 and COX-2 in spinal cord and decrease the pain score in rat with incision pain,which plays the preemptive analgesia effects.