Optimization of Hexadecylpyridinium Chloride Decontamination for Culture of Mycobacterium avium subsp. paratuberculosis from Milk |
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| Authors: | L. Bradner S. Robbe-Austerman D. C. Beitz J. R. Stabel |
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| Affiliation: | Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, USAa;USDA-APHIS, Veterinary Services, Ames, Iowa, USAb;Department of Animal Science, Iowa State University, Ames, Iowa, USAc;USDA-ARS, National Animal Disease Center, Ames, Iowa, USAd |
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| Abstract: | A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the culturing protocol, and such protocols should be optimized to ensure that low concentrations of MAP in milk can be detected. |
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