脾虚证模型大鼠血清中血管活性肠肽和胆囊收缩素的变化及四君子汤的干预作用

目的研究脾虚证模型大鼠血清中血管活性肠肽(VIP)和胆囊收缩素(CCK)的变化以及四君子汤的干预作用,探讨四君子汤调节大鼠胃肠动力的机制。方法采用饥饱失常加劳倦加破气耗气方法复制脾虚大鼠模型,将60只大鼠随机分为正常组、模型组、四君子汤组、莫沙必利组,用Elisa法测定每组大鼠血清中血管活性肠肽和胆囊收缩素的含量。结果模型组大鼠血清中血管活性肠肽含量(28.60±41.88)明显减少,与正常组(51.55±16.22)比较差异具有统计学意义(P<0.05);模型组大鼠血清中胆囊收缩素(3.55±2.53)含量明显升高,与正常组(2.05±0.67)比较差异具有统计学意义(P<0.05)。经治疗后,四君子汤组大鼠血清中血管活性肠肽(57.51±13.25)、莫沙必利组大鼠血清中血管活性肠肽(61.63±27.46)均较模型组显著升高(P<0.05),四君子汤组大鼠血清胆囊收缩素(2.29±0.81)较模型组显著降低(P<0.05)。结论四君子汤能调节脾虚证大鼠的胃肠激素,从而纠正其胃肠功能紊乱,这是四君子汤治疗脾虚证的作用机制之一。

Vasoactive intestinal peptide and cholecystokinin in serum of rat spleen deficiency model and the intervention effect of Sijunzi Decoction

Objective To investigate the changes of vasoactive intestinal peptide（ VIP） and cholecystokinin（ CCK） in serum of rat spleen deficiency model and intervention effect of sijunzi decoction,to explore the mechanism of gastrointestinal motor. Methods The rat model of spleen deficiency were established,and all the rats were divided randomly into four groups as following,the normal,model,sijunzi decoction and Mosapride. And the contents of VIP and CCK in serum of rats were measured with Elisa kit. Results The contents of VIP（ 28. 60 ± 41. 88） in rats serum of model group decreased evidently,compared with the normal group（ 51. 55 ± 16. 22）,the difference was statistically significant（ P〈0.05）. The contents of CCK（ 3. 55 ± 2. 53） increased evidently in rats serum of model group,compared with the normal group（ 2. 05 ± 0. 67）,the difference was statistically significant（ P〈0. 05）. After the treatment,the contents of VIP（57.51 ±13.25） in rats serum of sijunzi decoction group were higher than that in model group（ P0.05）,the contents of VIP（ 61. 63 ±27. 46） in rats serum of Mosapride group were also higher than that in model group（ P〈0. 05）. The contents of CCK（ 2. 29 ±0. 81） inrats serum of sijunzi decoction group were lower than that in model group（ P〈0. 05）. Conclusion The sijunzi decoction could regulate the gastrointestinal hormone of spleen deficiency rats,so as to correct tstomach and intestinal disorders,which was one of the mechanisms of sijunzi decoction treating spleen deficiency syndrome.