| 伽玛射线对人垂体瘤细胞细胞周期和抑癌基因p16表达的影响 |
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| 引用本文： | 陈春美,杨卫忠,王锐,石松生,王春华,陈建乐. 伽玛射线对人垂体瘤细胞细胞周期和抑癌基因p16表达的影响[J]. 中国医药导报, 2011, 8(10): 32-33 |
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| 作者姓名： | 陈春美 杨卫忠 王锐 石松生 王春华 陈建乐 |
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| 作者单位： | 福建医科大学附属协和医院神经外科,福建福州,350001;福建医科大学附属协和医院伽玛刀治疗中心,福建福州,350001;福建省神经外科研究所,福建福州,350001 |
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| 摘 要： | 目的：探讨不同剂量伽玛（γ）射线影响人垂体瘤细胞细胞周期和抑癌基因p16表达的变化。方法：采用机械分离法获得稳定生长垂体瘤原代培养细胞株,根据不同照射剂量分为4组照射组,分别接受中心剂量为2、5、8.22、13.33 Gy,设立对照组,剂量为0,照射后用流式细胞术检测细胞周期和凋亡率,Western Blot法检测p16蛋白的表达变化。结果：对照组和中心剂量2 Gy照射后细胞周期、凋亡率和c-myc蛋白表达无明显差异;中心剂量分别为5 Gy和8.22 Gy时细胞周期G0/G1和G2/M期细胞数量逐渐增多,细胞凋亡率升高,p16蛋白表达增多,与对照组比较,差异均有统计学意义（P〈0.05）;剂量为13.33 Gy时,S期细胞增多,p16蛋白表达减少。结论：一定剂量的伽玛射线能够改变细胞周期,促进垂体瘤细胞进入凋亡,抑癌基因p16在此过程中可能发挥促进垂体瘤细胞进入凋亡过程。
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| 关 键 词： | 垂体瘤 y射线 细胞周期 p16 |
Gamma-ray induced the change of human pituitary tumor cell cycle and the expression of tumor suppressor gene p16 |
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| CHEN Chunmei,YANG Weizhong,WANG Rui,SHI Songsheng,WANG Chunhua,CHEN Jianle. Gamma-ray induced the change of human pituitary tumor cell cycle and the expression of tumor suppressor gene p16[J]. China Medical Herald, 2011, 8(10): 32-33 |
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| Authors: | CHEN Chunmei YANG Weizhong WANG Rui SHI Songsheng WANG Chunhua CHEN Jianle |
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| Affiliation: | CHEN Chunmei1,2,3,YANG Weizhong1,WANG Rui1,SHI Songsheng1,WANG Chunhua1,CHEN Jianle1,3 1.Department of Neurosurgery,Union Hospital of Fujian Medicine University,Fuzhou 350001,China,2.Department of gama knife center,3.Fujian Institute of Neurosurgery |
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| Abstract: | Objective:To investigate the change of human pituitary tumor cell cycle and the p16 tumor suppressor gene expression after Gamma knife radiation.Methods:The primary pituitary tumor cells with stable growth were gained from a patient of pituitary tumor by mechanical separation,and were divided into 4 groups with the radiation center dose 2,5,8.22,13.33 Gy respectively.There was the control group without radiation.Flow cytometry was used to detect the cell cycle and apoptosis.The expression of p16 protein wer... |
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| Keywords: | p16 |
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