羟基喜树碱对人乳腺癌MCF-7细胞增殖及凋亡的影响

目的：探讨羟基喜树碱在体外对人乳腺癌MCF-7细胞增殖及凋亡的影响,为乳腺癌的临床用药提供实验依据。方法：将不同浓度（10μmol/L、50μmol/L、100μmol/L、200μmol/L）的羟基喜树碱作用于人乳腺癌MCF-7细胞48 h,并设不加羟基喜树碱溶液的MCF-7细胞作为对照组,用MTT法测定MCF-7细胞的增殖情况,用Hoechst33258染色检测MCF-7细胞的凋亡情况,以RT-PCR检测羟基喜树碱对MCF-7细胞Casepase-3 mRNA表达的影响。结果：与对照组比较,50μmol/L、100μmol/L及200μmol/L羟基喜树碱对细胞增殖均有抑制作用（P〈0.05、P〈0.01）,尤以200μmol/L的抑制作用最明显,其抑制率呈浓度依赖性增加。荧光显微镜观察显示,100μmol/L及200μmol/L羟基喜树碱处理组中可见典型的凋亡形态学改变。与对照组比较,各羟基喜树碱处理组中MCF-7细胞Caspase-3 mRNA表达水平均升高（P〈0.05、P〈0.01）,并呈浓度依赖性升高趋势。结论：羟基喜树碱可在体外抑制MCF-7细胞增殖,并通过Caspase-3途径诱导细胞凋亡,达到抑制肿瘤细胞生长的作用。

Effect of Hydroxycamptothecin on the proliferation and apoptosis of human breast cancer cell line MCF-7

Objective：To explore the effect of Hydroxycamptothecin（HCPT） on the proliferation and apoptosis of human breast cancer cell line MCF-7 in vitro,and to provide experimental basis for the clinical medication of breast cancer.Methods：HCPT with different concentrations（10 μmol/L,50 μmol/L,100 μmol/L,200 μmol/L） were used to treat MCF-7 cells for 48 hours,and MCF-7 cells without HCPT were served as the control group.MTT assay was applied to determine the proliferation of MCF-7 cells,Hoechst33258 staining was used to detect the apoptosis of MCF-7 cells,and RT-PCR was used to detect the effect of HCPT on the expression of Casepase-3 mRNA in MCF-7 cells.Results：Compared with the control group,50 μmol/L,100 μmol/L and 200 μmol/L HCPT showed obvious inhibitory effect on MCF-7 cells（P〈0.05,P〈0.01）,and the inhibitory effect of 200 μmol/L was the most obvious,the inhibitory rate showed a concentration-dependent tendency.Fluorescence microscope observation showed that typical apoptosis morphological changes could be observed in 100 μmol/L and 200 μmol/L HCPT treated cells.Compared with the control group,the expressions of Caspase-3 mRNA significantly increased in HCPT treated cells（P〈0.05,P〈0.01）,and it also showed a concentration-dependent tendency.Conclusion：HCPT can inhibit the proliferation of MCF-7 cells in vitro,and induce cell apoptosis by Caspase-3 pathway,and further achieve its role of inhibiting the growth of tumor cells.