| HPLC法测定活血通脉片中芍药苷的含量 |
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| 引用本文： | 徐晓弘,熊鑫.HPLC法测定活血通脉片中芍药苷的含量[J].中国医药导报,2011,8(7):59-60. |
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| 作者姓名： | 徐晓弘 熊鑫 |
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| 作者单位： | 大庆油田总医院药学部,黑龙江大庆,163001 |
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| 摘 要： | 目的：建立活血同脉片中芍药苷的含量测定方法。方法：采用HPLC对赤芍进行定量分析。用十八烷基硅烷键合硅胶为填充剂（4．6mm×250mm，5μm），SPD一10AVP检测器，流动相为甲醇-水（27：73），柱温为30℃，流速为0．8ml／min，检测波长为230nm。结果：芍药苷在0．98-7．35μg范围内呈良好的线性关系，平均回收率为97．81％，RSD=1．57％。结论：本法准确，重现性好，可用于活血通脉片的质量控制。
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| 关 键 词： | 活血通脉片 高效液相 芍药苷 含量测定 |
Content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC |
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| XU Xiaohong,XIONG Xin.Content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC[J].China Medical Herald,2011,8(7):59-60. |
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| Authors: | XU Xiaohong XIONG Xin |
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| Institution: | XU Xiaohong,XIONG Xin(Department of Pharmacy,General Hospital of Daqing Oil Field,Daqing 163001,China) |
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| Abstract: | Objective： To establish a method for the content determination of Paeoniflorin in Huoxue TongmaiTablets by HPLC. Methods： The radix Paeoniae rubra was quantitative analysed by HPLC. The C18（4.6 mm×250 mm, 5 μm） column was used. The mobile phases consisted of methanol and water （27：73）, the flow rate was 0.8 ml/min and the detection wavelength was at 230 nm. The temperature was 30℃. Results： The optimum linear relation of Peoniflorin was 0.98-7.35 μg, the average recovery rate was 97.52% with a RSD of 1.57%. Conclusion： The method is a accurate, recurring and easy to use assay for quality control. |
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| Keywords: | Huoxue Tongmai Tablets HPLC Paeoniflorin Content determination |
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