| HPLC法测定复方逍遥合剂中黄芩苷的含量 |
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| 引用本文： | 张武标,沈阳,刘兆康,王申东.HPLC法测定复方逍遥合剂中黄芩苷的含量[J].中国医药导报,2011,8(8):58-59. |
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| 作者姓名： | 张武标 沈阳 刘兆康 王申东 |
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| 作者单位： | 上海市儿童医院(上海交通大学附属儿童医院),上海,200040 |
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| 摘 要： | 目的：建立复方逍遥合剂中黄芩苷的含量测定方法。方法：采用HPLC法，色谱柱Calesil—C18（4．6mm×250mm，5μm），流动相：甲醇：0．1％磷酸（47：53），检测波长：280nm。结果：黄芩苷进样量在0．08～0．80μg范围内呈良好的线性关系（r=0．9999），低、中、高三种浓度的平均回收率为99．59％、100．22％、100．40％，RSD分别为0．75％、2．58％、0．86％。结论：本法简便、准确，重复性好，可用于该产品的质量控制。
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| 关 键 词： | 复方逍遥合剂 HPLC 黄芩苷 |
Content determination of Baicalin for Compound Xiaoyao Oral Solution by HPLC |
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| ZHANG Wubiao,SHEN Yang,LIU Zhaokang,WANG Shendong.Content determination of Baicalin for Compound Xiaoyao Oral Solution by HPLC[J].China Medical Herald,2011,8(8):58-59. |
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| Authors: | ZHANG Wubiao SHEN Yang LIU Zhaokang WANG Shendong |
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| Institution: | ZHANG Wubiao,SHEN Yang,LIU Zhaokang,WANG Shendong Children's Hospital of Shanghai,Shanghai Children's Hospital Affiliated of Shanghai Jiaotong University,Shanghai 200040,China |
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| Abstract: | Objective： To establish a method for determination the content of Baicalin in Compound Xiaoyao Oral Solution. Methods： Calesil-C18 column （4.6 mm×250 ram, 5 μm） was used, Mobile phase was methanol-0.1% phosphoric acid （47： 53）, Detection wavelength was 280 nm. Results： Baicalin sample size located 0.08-0.80 μg,and favourable linear correlation （r=0.999 9） was showed. The average recovery rates of low, moderate and high concentration were 99.59%, 100.22%, 100.40% with RSD of 0.75%, 2.58%, 0.86%, respectively. Conclusion： The method is convenient and accurate. Meanwhile, it also has good reproducibility, may be used to quality control of Baicalin. |
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| Keywords: | HPLC |
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