| HPLC测定6种蜂蜜中5-羟甲基糠醛的含量 |
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| 引用本文： | 龙光明,裘璐,马丽,郝小燕.HPLC测定6种蜂蜜中5-羟甲基糠醛的含量[J].数理医药学杂志,2012(6):643-645. |
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| 作者姓名： | 龙光明 裘璐 马丽 郝小燕 |
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| 作者单位： | 贵阳医学院药学院,贵阳550004 |
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| 基金项目： | 贵州省科技厅工业攻关项目（黔科合GY字[2009]3053） |
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| 摘 要： | 目的:建立蜂蜜中5-羟甲基糠醛(5-HMF)的含量测定方法。方法:利用高效液相色谱法测定6种蜂蜜中5-HMF的含量。结果:5-HMF在48.64～291.84ng范围内呈良好的线性关系,r=0.9999;平均回收率为100.02%,RSD为1.69%(n=6);6种蜂蜜中5-HMF的平均含量在0.0328～0.0398mg.g-1。结论:所建立的方法简便,重复性好,可用于检测蜂蜜中5-HMF的含量。
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| 关 键 词： | 蜂蜜 5-羟甲基糠醛 HPLC |
Determination of 5-HMF in Six Different Kinds of Honeys by HPLC |
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| Institution: | Long Guangming, et al (Guiyang Medical University, Guiyang 550004) |
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| Abstract: | Objective： To establish a HPLC method for the determination of 5-HMF in different types of honeys. Methods： HPLC method was applied to the determination of 5- HMF in honeys. Results： 5-HMF had a good linearity within the range of 48. 64-291.84 ng, r=0. 9999, The average recovery was 100.02%, RSD=1. 69%（n=6） ; The average contents of six types of honeys were 0. 0328 - 0. 0398 mg · g-1. Conclu- sions： The method is simple and repeatable , which can be used to determine the content of 5-HMF in hon- eys. |
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| Keywords: | honeys 5-HMF HPLC |
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