兔动脉粥样硬化中性白细胞变形性改变的机制

目的：探讨动脉粥样硬化（AS）的不同病理阶段中，中性白细胞变形性改变的分子机制。方法：动态检测兔AS前期、早期、中期和晚期的中性白细胞变形性、膜脂流动性、Ca2＋]i和Na＋－K＋ATPase活性。结果：（1）中性白细胞膜脂流动性和Na＋－K＋ATPase活性降低始于AS前期，而中性白细胞变形性明显减退和Ca2＋]i水平增高见于AS早期；（2）在AS进程中，中性白细胞变形性减退逐渐加重，与膜脂流动性和Na＋－K＋ATPase活性呈正相关，而与Ca2＋]i呈负相关。结论：本研究结果表示中性白细胞的变形能力取决于膜脂流动性、Na＋－K＋ATPase活性及Ca2＋]i水平，中性白细胞参与AS的发生和发展。

Molecular Mechanism of Reduced Deformability of Neutrophils at Various Stages of Atherosclerosis in Rabbits

Objective:The goal of this study was to investigate molecular mechanism of reduced deforma-bility of neutrophils at various stages of atherosclerosis(AS). Metkod: 40 rabbits were fed with cholesterol(lg d-1 each rabbit )for 12 weeks and atherosclerosis was induced. The membrane fluidity,Na -K ATPase ac-tivity,deformability and intracellular Ca2 concentration Ca2 ]i of neutrophils (PMNs)were assessed in AS rabbits as compared with 20 normal rabbits at the second,the fourth,the eighth and the twelfth week respec-tively. Results: The results were as follows: The Na - K ATPase activity and the membrane fluidity of PMNs were greatly decreased at the second week,but increased Ca2 ]i and decreased deformability of PMNs occur at the fouth week. These parameters change more and more from the second or the fourth week to the twelfth week. Conclusion:The results suggested that deformability of PMNs is dependent on membrane fluid-ity,Na -K ATPase activity and concentration of Ca2 ]i of PMNs,that PMNs take part in the happening and development of experimental atherosclerosis.