微血栓大鼠循环内皮细胞,血浆内皮素和一氧化氮测定的结果分析

目的：对微血栓动物模型行普通肝素和低分子量肝素的治疗，通过内皮细胞计数（CEC）、内皮素（ET）、一氧化氮（NO）三项指标观察分析其血管内皮细胞损害及肝素的治疗效果。方法：对18只wistar大鼠行尾静脉注射高分子右旋糖苷（高右），制成微血栓动物模型。其中12只在高右基础上分别予普通肝素（普肝组，n＝6）和低分子量肝素（低肝组，n＝6）治疗。其余6只不治疗。另以5只wistar大鼠行尾静脉注射生理盐水为正常对照组（对照组，n＝5）。对上述各组作循环内皮细胞计数（CEC）、血浆内皮素（ET）及一氧化氮（NO）测定。结果：高右组CEC、ET高于对照组和治疗组（P＜0．05），NO则低于对照组和治疗组（P＜0．05）。治疗组的上述结果均有明显恢复至对照组的趋势。结论：微血栓大鼠有血管内皮损伤，普通肝素及低分子量肝素不仅能抗微血栓形成，且可促进血管内皮细胞功能恢复，其中以低分子量肝素为优。

Assay of Circulating Endothelial Cell, Endothelin and Nitric Oxide Determination in Microthrombotic Rats

Objective:To study the injury of circulating endothelial cell and the curative effect of the heparin in microthrombotic rats. Method: 23 Wistar rats were put into 4 groups. Intravenous injection of saline was for the control group (Group A n=5); Intravenous injection of high molecular dextran (HMD) was for experimental group (Group B n=6); low molecular heparin (LMH) and heparin (H) were injected respectively in two groups (C1,n= 6 C2,n=6 ). To count CEC, measure ET and NO according to the manual. Result: The number of CEC and ET in Group B was increased more significantly than Group A and C1, C2 groups (P< 0.05). The results mentioned in C1and C2 groups were recovered to Group A. Conclusion: The circulating endothelial cells were injuried in microthrombotic rats. Two kinds of heparin were antithrombotic. The curative effect of the low molecular heparin was better.