MR扩散加权成像在氟尿嘧啶治疗小鼠皮下移植肝癌疗效评价中的价值

目的:探讨磁共振DWI成像技术在氟尿嘧啶治疗小鼠皮下移植肝癌疗效评价中的作用.方法:将小鼠随机分为两组,制备小鼠H22皮下种植肝癌模型.种植成功第5天后,A组隔天给予氟尿嘧啶腹腔注射,B组给予蒸馏水灌胃.第15天进行MR检查,测量肿瘤组织的表观扩散系数(ADC).检查完毕后处死小鼠,测量血清中血管生成因子(VEGF)水平;取瘤体及脾脏分别称重,计算肿瘤指数及脾脏指数;检测新鲜肿瘤组织肝癌细胞凋亡的DNA百分比.将两组小鼠的ADC值及实验室数据行t检验,分析两组小鼠ADC值与各实验室结果的相关性.结果:在扩散敏感因子(b)=600s/mm~2及1200s/mm~2的DWI图像中,肝癌多表现为高信号,其内的坏死部分为低信号.A组、B组肿瘤实质部分的ADC值分别为:780±122mm~2/s、596.50±80.17mm~2/s;1421 ±160mm~2/s、1232.804-85.02mm~2/s,两者具有统计学差异(P<0.05).肿瘤实质成分的ADC值与血清中VEGF水平、肝癌细胞凋亡的DNA百分比、处死后称得的瘤体及脾脏重量存在相关性.结论:ADC值可以反映氟尿嘧啶对肿瘤生长抑制的情况,MR DWI技术及ADC值在氟尿嘧啶治疗小鼠皮下移植肝癌疗效评价方面具有可行性.

Value of MR Diffusion Weighted Imaging in Evaluating Therapeutic Effect of Fluorouracil in Rats Transplanted by Hepatocellular Carcinoma Subcutaneously

Purpose: To investigate the role of DWI technology in evaluating the effect of fluorouracil in H22 hepatoma rat model. Methods: A total of 20 rats were divided into 2 groups randomly. The H22 hepatocellular carcinoma rat models were established. Five days after successful cultivation, group A was treated by injecting fluorouracil intraperitoneally once of 2 days, group B was distilled water orally. Rats in each group were performed with MRI after 15 days. The apparent diffusion coefficient (ADC) of the tumor was measured and the images were observed. The rats were sacrificed after the exams were finished. The serum vascular endothelial growth factor(VEGF) level and the apoptosis of tumor cells were measured. The tumors and spleens were weighted to calculate tumor index and spleen index. The ADC value and the laboratory data of the two groups were compared with t test. The relationship of ADC value and the laboratory data was evaluated. Results: In DWI with b value = 600 and 1200mm~2/s, hepatocellular carcinoma displayed high signal, and the signal of necrosis was low. ADC values in solid parts of tumor in group A and group B were 780 ± 122mm~2/ s, 596.50 ± 80.17mm~2/ s and 1421 ± 160mm~2/ s, 1232.80 ± 85.02mm~2/s, respectively, and with significant difference (P < 0.05) . The ADC value of the parenchy-matous tissue of the tumor was correlation with the serum VEGF level, tumor cell apoptosis, and the weight of the tumor and spleen. Conclusion: ADC value can reflect the inhabitation of fluorouracil totumor growth. DWI technology and ADC value is a feasible method in evaluating of the effect of fluo-rouracil in the treatment of hepatocellular carcinoma transplanted subcutaneously in rats.