不同临床病理特征的食管鳞癌组织中上皮间质转化相关蛋白的表达

目的 探讨不同临床病理特征的食管鳞癌组织中上皮间质转化(EMT)相关蛋白的表达情况.方法 100例食管鳞癌患者,根据肿瘤芽数不同分为低肿瘤芽组(芽数<5个,n=40)及高肿瘤芽组(芽数≥5个,n=60),比较两组肿瘤组织中EMT相关蛋白上皮间质转化因子(Twist)、上皮性钙黏附因子(E-cadherin)以及β-连环蛋白(β-catenin)的表达情况,以及在不同临床病理特征中各相关蛋白的表达情况.结果 高肿瘤芽组Twist阳性表达率高于低肿瘤芽组,而E-cadherin及β-catenin阳性表达率低于低肿瘤芽组(均P<0.05).相较于无淋巴结转移者,有淋巴结转移者癌组织的Twist及β-catenin阳性表达率高,E-cadherin阳性表达率低(均P<0.05);随着疾病进展,Twist的阳性表达率降低(P<0.05);随着分化程度升高,E-cadherin的阳性表达率降低,β-catenin的阳性表达率升高(均P<0.05);随着肿瘤浸润深度增加,Twist、E-cadherin及β-catenin阳性表达率均升高(P<0.05).结论 Twist、E-cadherin以及β-catenin表达与食管鳞癌肿瘤芽形成、淋巴结转移以及浸润深度具有一定联系.

Expression of epithelial-mesenchymal transitions associated protein in esophageal squamous cell carcinoma tissues with different clinicopathological features

Objective To investigate the expression of epithelial-mesenchymal transitions ( EMT) associated protein in esophageal squamous cell carcinoma tissues with different clinicopathological features .Methods A total of 100 patients with esophageal squamous cell carcinoma were divided into low tumor budding group (the number of buds <5,n=40) and high tumor budding group (the number of buds≥5,n=60) according to the number of tumor buds.The EMT associated protein epithelial mesenchymal conversion factor (Twist), epithelial cadherin factor(E-cadherin) andβ-catenin expressions were compared between the two groups,and the expressions of the associated proteins mentioned above with different clinicopathological features were also compared .Results The positive expression rate of Twist was higher while the positive expression rates of E-cadherin andβ-catenin were lower in the high tumor budding group compared to the low tumor budding group(all P<0.05).The positive expression rates of Twist and β-catenin were higher while the positive expression rate of E-cadherin was lower in patients with lymph node metastasis compared to patients without lymph node metastasis (all P<0.05).The positive expression rate of Twist decreased with the progression of the disease(P<0.05).The positive expression rate of E-cadherin increased while the positive expression rate ofβ-catenin decreased with the increasing differentiation grades(all P<0.05).The positive expression rates of Twist ,E-cadherin and β-catenin increased with the increasing tumor infiltration depth(P<0.05).Conclusion The expressions of Twist,E-cadherin and β-catenin may be related to tumor budding ,lymph node metastasis and infiltration depth in esophageal squamous cell carcinoma .