| 大鼠局灶性脑缺血后再灌注期caspase—9 mRNA及Apaf—1mRNA表达的动态变化 |
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| 引用本文： | 朱炬,王纪佐. 大鼠局灶性脑缺血后再灌注期caspase—9 mRNA及Apaf—1mRNA表达的动态变化[J]. 中国临床神经科学, 2003, 11(1): 23-26 |
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| 作者姓名： | 朱炬 王纪佐 |
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| 作者单位： | 天津医科大学第二医院神经内科,300211 |
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| 摘 要： | 目的探讨大鼠局灶性脑缺血后再灌注期caspase-9 mRNA及Apaf-1 mRNA表达的动态变化.方法采用线栓法制作大鼠局灶性脑缺血后再灌注模型,以逆转录聚合酶链式反应(RT-PCR)技术检测caspase-9 mRNA及Apf-1 mRNA的表达.结果缺血2 h后再灌注,缺血皮质中caspase-9 mRNA的表达在再灌注后24h达高峰,48h仍保持高水平,而Apaf-1 muRNA的表达无明显改变.结论局灶性脑缺血后再灌注48h内caspase-9 mRNA表达增强.
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| 关 键 词： | 局灶性脑缺血 再灌注 caspase-9 mRNA Apaf-1 mRNA RT-PCR |
| 文章编号： | 1008-0678(2003)01-023-04 |
| 修稿时间： | 2002-09-02 |
Changes of Caspase-9 mRNA and Apaf-1 mRNA Expression in Reperfusion Stage Following Focal Cerebral Ischemia in Rat |
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| ZHU Ju,WANG Ji-Zuo. Changes of Caspase-9 mRNA and Apaf-1 mRNA Expression in Reperfusion Stage Following Focal Cerebral Ischemia in Rat[J]. Chinese Journal of Clinical Neurosciences, 2003, 11(1): 23-26 |
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| Authors: | ZHU Ju WANG Ji-Zuo |
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| Affiliation: | ZHU Ju,WANG Ji-Zuo Department of Neurology,The Second Hospital of Tianjin Medical University,Tianjin 300211,China |
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| Abstract: | Aim:To investigate the change of caspase-9 and Apaf-1 expression in reperfusion stage following focal cerebral ischemia. Methods: The reperfusion model following focal cerebral ischemia in rat was made by thread embolish of MCA and the expression of caspase-9 and Apaf-1 mRNA was detected by using RT-PCR technique. Results: During reperfusion after 2 h of ischemia, the expression of caspase-9 mRNA changed with time in the ischemic cortex, while the expression remained at high level after 48h of reperfusion and with peak at 24 h, the expression of Apaf-1 mRNA did not change significantly in the ischemic cortex. Conclusion: Caspase-9 mRNA expression level is high during reperfusion after cerebral ischemia. |
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| Keywords: | focal cerebral ischemia reperfusion caspase-9 mRNA Apaf-1 mRNA RT-PCR |
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