25味珊瑚丸载药纳米颗粒的生物效应研究

目的　探讨25味珊瑚丸载药纳米颗粒（Ershiwuwei Shanhu’ Pill , ESP)于体内外的生物效应。 方法　将ESP纳米颗粒与SH-SY5Y细胞共培养8 h，荧光示踪与透射电镜(Transmission electron microscopy , TEM) 观察其在细胞吸收分布情况。大鼠随机分为空白对照组和用药组，分别通过尾静脉注射生理盐水或ESP， HE染色观察肝脏形态的变化，旷场实验(Open field test)评估大鼠兴奋度变化，扫描电镜(scanning electron microscope , SEM)观察SH-SY5Y细胞在25味珊瑚丸不溶组分上的生长情况。 结果　25味珊瑚丸纳米颗粒出现在各种细胞器中，出现破碎的细胞核。体内实验中，用药组大量肝细胞液化坏死，与模型组相比，用药组大鼠动物总运动距离显著增加（P<0.05），细胞可在25味珊瑚丸不溶组分生长良好。 结论　25味珊瑚丸载药纳米颗粒可能导致大鼠焦虑并具有强烈的肝毒性。

Study on biological effect of nanoparticles from Ershiwuwei Shanhu’ Pill

Objective　To explore the biological effect of drug-loaded nanoparticles from Ershiwuwei Shanhu’Pill.　Methods　The ESP drug-loaded nanoparticles were incubated with SH-SY5Y cells for 8hrs. Cellular uptake and intracellular distribution was observed by immunofluorescent staining assays. Intracellular translocation was tracked by transmission electron microscopy (TEM). Rats were randomly divided into a model group and an ESP-treated group, being injected with normal water or drug-loaded nanoparticles through tail vein, respectively. HE staining was used to observe liver pathological change. Changes of excitatory was assessed by open field test. SH-SY5Y development on the ESP insoluble components were measured by scanning electron microscope (SEM). Results The drug-loaded nanoparticles could fill the whole cells and the cellular nucleus was fragmentized. In vivo experiments, the liver cells in the treatment group had liquefactive necrosis. Compared with the model group, total distance was significantly improved in ESP-treated group (P＜0.05). Cells regularly growing on the insoluble component exsited in ESP. Conclusion　Drug-loaded nanoparticles from ESP may lead to anxiety and strongliver toxicity of rats.