曲古霉素A增强抗肿瘤药物对膀胱癌T24细胞的杀伤作用

目的观察HDAC抑制剂曲古霉素A(TSA)和针对DNA的抗癌药物联用对膀胱癌T24细胞的杀伤作用。方法用MTT法测定TSA单用及分别与ADM、MMC和DDP联用对T24细胞的抑制率,用金氏公式法判断联合用药的效果。结果TSA分别与ADM、MMC、DDP联合用药对T24细胞的抑制率均随着浓度的增加而明显增加,TSA与MMC联用的协同作用最明显,而TSA与ADM和DDP在中低剂量下联用亦表现为协同作用。结论HDAC抑制剂TSA可明显增强针对DNA的抗癌药物对膀胱癌细胞的杀伤作用,有希望应用于晚期膀胱癌的化疗方案中。

Trichostatin A inhances cytotoxic activity of antineoplastics on bladder caner cell T24

Aim To investigate the cytotoxic activity of HDAC inhibitor Trichostatin A (TSA)combined with anticancer drugs targeting DNA on T24 bladder cancer cell line.Methods MTT assay was used to detect the inhibitory rate of TSA alone or combined with ADM, MMC and DDP respectively on T24 bladder cancer cell in cancer cell proliferation by administering TSA alone or combined with ADM, MMC and DDP respectively.Jin′s equation was used to evaluate the efficacy of drug combination. Results The growth inhibitory rate of TSA combined with ADM, MMC and DDP respectively was in a concentration-dependent manner. The synergism of TSA combined with MMC was most significant. When administered in lower or moderate concentration, TSA combined with ADM or DDP in lower or moderate concentration demonstrated synergic effect too.Conclusion HDAC inhibitor TSA enhances the cytotoxic activity of anticancer drugs targeting DNA on bladder cancer cells and is promising to be used in chemotherapeutic regimens for advanced bladder cancer.