Glucose transport in Leishmania donovani promastigotes |
| |
| Authors: | Dan Zilberstein Dennis M. Dwyer |
| |
| Affiliation: | Cell Biology and Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institute of Health, Bethesda, MD 20205, U.S.A. |
| |
| Abstract: | Mid-log phase Leishmania donovani promastigotes accumulated 2-deoxy-d-glucose (2-DOG) via a carrier mediated transport system, maintaining an apparent Km of 24.4 μM and a Vmax of 3.12 nmol mg?1 protein min?1. d-Glucose but not l-glucose competitively inhibited the 2-DOG transport with an apparent Ki of 18.7 μM. Transport of 2-DOG was inhibited by 2,4-dinitrophenol and NaN3. The parasites maintained a 2-DOG gradient of at least 79 fold across the surface membrane, demonstrating the active nature of the transport system. |
| |
| Keywords: | Promastigotes Carrier-mediated transport Active transport DNP, 2,4-dinitrophenol |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
