BALB/c小鼠黑色素瘤B 16移植肿瘤模型的建立

[目的]建立宜于进行早期观察及测量的小鼠黑色素瘤模型.[方法]将不同数量的处于对数生长期的B16细胞株黑色素瘤细胞接种于BALB/c小鼠背部及足垫皮下,建立移植肿瘤动物模型.观察小鼠肿瘤的生长特点,应用病理学和免疫组织化学染色方法进行鉴定,归纳总结接种细胞数与肿瘤发生率的关系及肿瘤生长对小鼠生活状态的影响.[结果]接种5d后BALB/c小鼠皮肤浅表部位相继出现黑色的点或线状改变,发展为结节状改变直至肿瘤破溃、出血、死亡或破溃、出血、结痂、愈合,部分小鼠出现肿瘤消退.在接种细胞数大于5×105个时BALB/c小鼠的肿瘤模型全部建立成功.[结论]B16细胞株黑色素瘤细胞接种于BALB/c小鼠的肿瘤模型成功率与接种细胞数成正比,它具有建立肿瘤模型成功率高,观察及测量方便等优点,接种部位以背部皮下为宜.

Establishment of the neoplasm transplantation with B16 melanoma in BALB/c mice

OBJECTIVE To establish neoplasm transplantation models of melanoma in BALB/c mice.METHODS B 16 melanoma cells in logarithmic growth phase were inoculated into BALB/c mice's back hypodermis and feet with different concentration to establish neoplasm transplantation of melanoma.The growth characteristics of transplanted melanoma in mice were observed and the tumor properties were identified by histopathology and immunohistochemistry.The relations between the number of B 16 cells inoculated and tumorigenetic rate,and the influence of tumorigenesis for mice were observed.RESULTS Five days later,black spots and linear changes were gradually seen in BALB/c mice's back skin,feet or ankle,and then they developed to tubercles,ulceration,hemorrhage and death.The tumorigenetic rate in inoculating with 5×105 B 16 cells was near 100%.CONCLUSION The tumorigenetic rate has a positive relation to the number of inoculated B 16 cells,and is high in BALB/c mice inoculated the B 16 cells into back hypodermis.