绵茵陈提取液的大孔树脂静态吸附工艺影响因素考察

目的:考察绵茵陈提取液的静态吸附工艺的影响因素,以确定其大孔吸附树脂工艺路线和参数。方法:以吸光度和绿原酸的比吸附量为指标,静态吸附法考察9种树脂的吸附效果和绵茵陈提取液在F树脂的吸附时间曲线;采用析因实验设计,静态吸附法考察上柱药液浓度和pH对绿原酸吸附的影响;以绵茵陈提取液和洗脱液的HPLC指纹图谱为指标,考察其精制效果。结果:不同树脂对绵茵陈提取液中成分的吸附效果有差异;绵茵陈提取液在F树脂的吸附平衡时间为6 h;上柱药液浓度和pH是影响吸附的重要因素。验证实验表明该工艺在有效保留HPLC指纹图谱成分的同时,使提取液的得膏率降低到2.5%。结论:该结果可为绵茵陈提取液的大孔树脂精制工艺路线的确定提供参考。

Influencing factors of static adsorbing process of extract of Herba Artemisiae scopariae with macroporous resin

AIM: To study the influencing factors of static adsorpting process of extract of Herba Artemisiae scopariae with macroporous resin,and determine the refinement process. METHODS: The absorbances and the adsorption quantity of caffeotannic acid were used as indexes,the adsorption effect of 9 kinds of macroporous resin and static adsorption curve of F resin;density and pH of the extract liquor were investigated by use of factorial experiment;HPLC fingerprint of extract of Herba Artemisiae scopariae were evaluated the adsorption effect.(RESULTS:) The adsorpting effect were different among types of macroporous resin,and the adsorpting equilibrium time was 6 h in the use of F resin;Density and pH of the extract liquor are important factors of adsorption;The experiment indicated that decreased liquor by this process were important factors of adsorption;The experiment indicated that this process decreased the yield of extract by 2.5%. CONCLUSION: The result can offer information about the determination of refinement process of extract of Herba Artemisiae scopariae with macroporous resin.