胶质瘤细胞与星形胶质细胞骨架荧光成像比较分析

目的 探讨C6胶质瘤细胞与侵袭性相关的骨架构筑特点。方法 利用免疫荧光技术对C6胶质瘤细胞及星形胶质细胞的骨架成分进行成像及比较分析，流式细胞仪检测两种细胞F-actin的荧光强度。结果 C6胶质瘤细胞的三种骨架成分呈网架结构，以核为中心向外周放射，边界不齐、毛糙，此外，微丝发达密集，中间丝呈高度网络结构。星形胶质细胞三种骨架成分呈网架结构，但细胞边界整齐，微丝稀疏，中间丝发达密集，呈栏栅状排列。C6胶质瘤细胞内F-actin的荧光强度明显强于星形胶质细胞内F-actin的荧光强度，p＜0.01。结论 C6胶质瘤细胞骨架呈网架结构，边界不齐、毛糙，微丝发达密集，中间丝呈高度网络结构，可能均与侵袭性相关的特征结构表现, C6胶质瘤细胞含有丰富的F-actin与侵袭性有关。

The comparison and analysis of fluorescence image of cytoskeleton on glioma cells and horizontal cells

Objective The purpose of this article is to explore the characteristics of cytoskeleton structure related to invasion of C6 glioma cells. Methods The image of cytoskeleton components of glioma cells and horizontal cells which were gained by immunofluorescence technique were compared and analyzed. At the same time, the fluorescence intensity of F-actin within two kinds of cells were detected by flow cytometry. Results Three kinds of cytoskeleton components of C6 glioma cells presented wire frame structure with radiation from nucelus to periphery and irregularity and coarse of cell boundary. The plentiful microfilaments and the sufficient network structure of intermediate filaments of C6 glioma cells could be seen. However, rare microfilaments and plentiful intermediate filaments with barrier like arrangement were shown in horizontal cells. Meanwhile, the fluorescence intensity of F-actin within C6 glioma cells were more stronger than them within horizontal cells. It shown significant difference. Conclusion The wire frame structure of cytoskeleton with irregularity and coarse of cell boundary of C6 glioma cells possessing plentiful microfilaments and the sufficient network structure of intermediate filaments were possible characteristic structures related to invasion. The redundant F-actin of C6 glioma cells were related to their invasion.