清肺口服液对3I、7b型腺病毒感染人胚肺成纤维细胞Fas、FasL蛋白表达的影响

目的:探讨清肺口服液含药血清对腺病毒3I、7b感染的人胚肺成纤维细胞Fas、FasL表达的影响。方法:细胞分为正常细胞组、病毒对照组、空白血清组、利巴韦林组和含药血清组,除正常细胞组外,其余4组均以3I、7b型腺病毒分别攻击,并分别加入相应药物至各组细胞中;用流式细胞仪检测比较各组细胞Fas、FasL蛋白的表达。结果:病毒对照组较正常细胞组细胞中Fas、FasL表达升高,含药血清组比病毒对照组细胞Fas、FasL表达降低。结论:腺病毒感染可使人胚肺成纤维细胞Fas、FasL蛋白表达增高;清肺口服液可降低Fas、FasL蛋白表达,从而抑制由腺病毒引发的细胞凋亡,这可能是其抗病毒作用的机制之一。

Effect of Qingfei Oral Liquid on Protein Expression of Fas and FasL of Human Embryonic Lung Fibroblast Induced by Adenovirus Type 3I and 7b

Objective: To explore the effect of Qingfei oral liquid(QOL) contained serum on Fas/FasL protein expression of human embryonic lung fibroblast induced by adenovirus type 3I and 7b.Methods: The cells were divided into 5 groups,the normal cells group(NCG),the virus control group(VCG),the blank serum group(BSG),the ribavirin group(RVG) and the QOL contained serum group(QSG).All the cells except those in the NCG were challenged by adenovirus type 3I and 7b and treated with correspondent medicine.The protein expression of Fas and FasL were detected with flow cytometric cell cycle analysis and compared among groups. Results: Expression of Fas and FasL in VCG were significantly higher,while those in QSG were significantly lower than those in VCG(P<0.01).Conclusion: Adenovirus infection can increase the protein expression of Fas and FasL of human embryonic lung fibroblast.QOL can decrease the protein expression of Fas and FasL,which maybe one of the mechanisms of its antiviral effect.