健康供者特征对rhG-CSF预激的骨髓采集物CD34+细胞和T细胞亚群的影响

目的 探讨健康供者特征对rhG-CSF预激的骨髓(G-BM)采集物造血和免疫细胞成分的影响.方法 150名健康供者体内应用rhG-CSF 5 μ·kg-1·d-1连续4～5 d,第4,5天采集骨髓和外周血,用流式细胞术测定G-BM采集物中的CD3+、CD3+CD4+、CD3+CD8+、CD14+、CD34+细胞以及CD3+CD4-CD8-调节性T细胞的数量,并分析供者性别、年龄、体重、是否妊娠等特征对G-BM成分的影响.结果 150名健康供者G-BM所含有核细胞,淋巴细胞,CD3+、CD3+CD4+、CD3+CD8+、CD14+、CD34+细胞以及CD3+CD4-CD8-调节性T细胞的中位值分别为31 050(12 200～58 100)、2122(506～6618)、1344(307～4791)、692(145～3038)、616(112～2575)、986(265～2958)、63(11～505)和83(9～390)/μl.年龄≤38岁的供者G-BM中的有核细胞、CD34+细胞以及CD3+ CD4-CD8-调节性T细胞的数量分别为33800(18600～57100)、76(22～505)和97(11～380)/μl,显著高于年龄＞38岁的供者分别为28 000(12 200～58 100)、49(11～220)和65(9～389)/μl],P值分别为＜0.05,＜0.001和0.001.外周血单核细胞计数＞0.37×109 /L的供者G-BM中有核细胞的数量33550(12 200～57 100)/μl]显著高于≤0.37×109 /L的供者27 150(13 800～58 100)/μl](P=0.005).多因素分析显示年龄和外周血单核细胞是G-BM中有核细胞采集量的影响因素HR值分别为0.41和2.87;P值分别为0.01和0.003];年龄是G-BM中CD34+细胞采集量的唯一影响因素(HR=0.26;P值=0.001);年龄还是G-BM中CD3+ CD4-CD8-调节性T细胞数量的影响凶素HR值为0.31;P=0.001].结论 年龄是影响G-BM中有核细胞、CD34+细胞和CD3+ CD4-CD8-调节性T细胞采集数量的因素,年龄≤38岁的供者更易采集满足临床需要的有核细胞和CD34+细胞;外周血单核细胞＞0.37×109 /L的供者采集的G-BM含有较多数量的有核细胞.

CD34+ cells and T cell subsets in recombinant human granulocyte colony-stimulating factor primed bone marrow grafts from donors with different characteristics

Objective To investigate the cell composition in granulocyte colony-stimulating factor (rhG-CSF)primed bone marrow grafts(G-BM)from donors with different characteristics.Methods One tive days.Bone marrow and peripheral blood stem cells were harvested at day 4 and day 5.The number of CD3+,CD3+ CD4+,CD3+ CD8+,CD14+,CD34+ and CD3+ CD4-CD8-cells were determined by multicolor flow cytometry.Cell composition of G-BM from donors with different characteristics,including sex,age,weight,pregnancy were analysed.Results The absolute number of nuclear cells(NCs),lymphocytes,CD3+,CD3+ CD4+ ,CD3+ CD8+,CD14+,CD34+ and CD3+ CD4-CD8-cells in G-BM were 31 050 (12 200-58 100),2122(506-6618),1344(307-4791),692(145-3038),616(112-2575),986 (265-2958),63(11-505)and 83(9-390)per microliter,respectively.The number of NCs33 800 (18 600-57 100)],CD134+ cells76(22-505)],and CD3+ CD4-CD8-regulatory T cells97(11-380)]harvested from younger donom(aged≤38 years)were significantly higher than those from older ones (aged＞38)28 000(12 200-58 100),49(11-220),and 65(9-389)per microliter(P=0.027,＜0.001 and=0.001,respectively)].Compared with that in donors with peripheral blood monocytes (PBMs)≤0.37×109 /L,higher number of NCs in G-BM27 150(13 800-58 100) vs 33 550(12 200-57 100),P=0.005 ] were collected in donors with PBMs＞0.37×109 /L.Multivariate analysis showed that donors age(≤38 vs ＞38 years;P=0.01)and monocyte number in peripheral blood(≤0.37×109 /L vs ＞0.37×109 /L;P=0.003)were factors predicting for NC yields,and donors age(≤38 vs ＞38 years;P＜0.001)was factor predicting for yields of CD34+ cells(P＜0.001)and CD3+ CD4-CD8- regulatory T cells (P=0.001)collection.Conclusion Donor age is a factor for the yields of NCs,CD34+ cells,and CD3+ CD4-CD8-regulatory T cells collection in G-BM,and donor's PBMs more than 0.37×109 /L,is another factor affecting NCs harvests.