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| 抗哇巴因单克隆抗体的制备及初步鉴定 | |
| 引用本文： | 马可为,袁育康,范桂香,吕卓人,王军阳,李建国,任会勋.抗哇巴因单克隆抗体的制备及初步鉴定[J].西北药学杂志,2005,20(5):209-211. |
| 作者姓名： | 马可为 袁育康 范桂香 吕卓人 王军阳 李建国 任会勋 |
| 作者单位： | 1. 世界图书出版西安公司 2. 西安交通大学医学院免疫学教研室,陕西,西安,710061 3. 西安交通大学第一医院,陕西,西安,710061 |
| 摘 要： | 目的制备抗哇巴因单克隆抗体。方法以半抗原哇巴因与蛋白质载体卵清蛋白(OVA)的耦联物为抗原,免疫BALB/C小鼠,取脾细胞与SP2/0细胞融合,获得两株分泌抗哇巴因的单克隆抗体的细胞株(OUA1,OUA2)。结果两株细胞染色体数目均为100条左右,证实为杂交瘤细胞;所分泌抗体为小鼠IgG1亚类;小鼠腹水效价测定分别为5×106,8×106;和地高辛交叉反应率分别为1.342%和2.323%;ELISA相加试验表明,两株单克隆抗体可能针对同一抗原决定簇。结论成功制备出小分子物质哇巴因的单克隆抗体。 |
| 关 键 词： | 内源性哇巴因 单克隆抗体 杂交瘤 |
| 文章编号： | 1004-2407（2005）05-0209-03 |
| 收稿时间： | 2005-02-28 |
| 修稿时间： | 2005年2月28日 |
Preparation and preliminary identification of monoclonal antibody against Ouabain |
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| MA Ke-wei,YUAN Yu-kang,FAN Gui-xiang,Lǖ Zhuo-ren,WANG Jun-yang,LI Jian-guo,REN Hui-xun.Preparation and preliminary identification of monoclonal antibody against Ouabain[J].Northwest Pharmaceutical Journal,2005,20(5):209-211. | |
| Authors: | MA Ke-wei YUAN Yu-kang FAN Gui-xiang Lǖ Zhuo-ren WANG Jun-yang LI Jian-guo REN Hui-xun |
| Abstract: | Objective To prepare and identify the anti-ouabain monoclonal antibody. Methods: Spleen cells were prepared from BALB/C mice which was immunized with hapten ouabain combining with protein (OVA),and then were fused with SP2/0 cells. Two hybridoma cell strains were acquired(OUA1,OUA2). Results: Both Strains chromosomal numbers were about 100 and two McAbs types were IgG_1 .Ascites titers were 5×10~6 and 8×10~6 . Crossing-reaction rates with digoxin were 1.342% and 2.323% respectively. ELISA additive test suggested that two McAbs were against the same epitope. Conclusion: The anti-ouabain monoclonal antibody was successfully prepared. |
| Keywords: | Endogenous ouabain Monoclonal antibody Hybridoma |
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