三七总皂甙对大鼠移植肝缺血再灌注损伤的保护作用

摘要：目的:探讨三七总皂甙预处理对大鼠移植肝缺血再灌注损伤的保护作用。方法:建立大鼠原位肝移植模型，然后分为三七预处理组（P组）、对照组（N组）和假手术组（S组）3组，取血检查ALT，AST；用免疫组化法检测caspase-3和TNF-α的表达，TUNEL法检测肝细胞凋亡，行肝组织病理检查。结果:鼠肝移植模型N组的血ALT，AST数值、肝细胞中caspase-3和TNF-α的表达及肝细胞的凋亡均明显高于P组，差异均有显著意义（P<0.05）。结论:肝细胞凋亡加重移植肝缺血再灌注损伤，三七总皂甙预处理对大鼠肝移植缺血再灌注损伤有保护作用。

The protective effect of panax notoginseng saponins on the transplanted liver during ischemia-reperfusion injury in rat with orthotopic liver transplantation

Abstract:Objective:To investigate the protective function of panax notoginseng saponins(PNS) on grafted liver during ischemia-reperfusion in rat with orthotopic liver transplantation. Methods:After establishing rat orthotopic liver transplantation model, the rats were randomly divided into three groups:the experimental group(P),the control group(N), and sham operation group(S). The levels of serum ALT and AST were determined,the hepatic pathohistological changes were observed,the expression of caspase-3 and TNF-α were examined by immunohistochemistry, and the apoptotic hepatocytes in grafted liver were detected by TUNEL method. Results:The levels of ALT, AST, and the expression of caspase-3 and TNF-α,and apoptotic cells in grafted liver were significantly higher in the control group than that in the experimental group. Conclusions:PNS can protect the grafted liver from injury during ischemia-reperfusion in rat orthotopic liver transplantation.