| RP-HPLC法测定复方五味子胶囊中五味子甲素的含量 |
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| 引用本文： | 李慧,肖佳尚. RP-HPLC法测定复方五味子胶囊中五味子甲素的含量[J]. 中国实用医药, 2008, 3(21): 36-37 |
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| 作者姓名： | 李慧 肖佳尚 |
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| 作者单位： | 1. 广州市药品检验所六分所,510800
2. 广州市药品检验所三分所 |
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| 摘 要： | 目的建立测定复方五味子胶囊中五味子甲素含量的高效液相色谱法。方法采用Kro-masilC18（250mm×4.6mm,5μm）的色谱柱;以甲醇-0.1%冰醋酸水溶液（82∶18）为流动相;流速：1ml/min;检测波长：254nm;柱温为30℃。结果五味子甲素在0.1137～0.4547mg/ml范围内与峰面积呈良好的线性关系（r=0.9992,n=6）,平均加样回收率为99.01%（RSD=1.1%,n=6）。结论HPLC法测定复方五味子胶囊中五味子甲素含量方法简便、准确、可靠。
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| 关 键 词： | 高效液相色谱法 复方五味子胶囊 五味子甲素 |
Determination of Deoxyschizandrin in Compound Wuweizi Capsules by RP-HPLC |
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| LI Hui,XIAO Jia-shang. Determination of Deoxyschizandrin in Compound Wuweizi Capsules by RP-HPLC[J]. China Practical Medical, 2008, 3(21): 36-37 |
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| Authors: | LI Hui XIAO Jia-shang |
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| Affiliation: | LI Hui, XIAO Jia- shang ( 6 Branch Institute for Drug Control of Guangzhou city, Guangzhou 510800, China) |
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| Abstract: | Objective To set up a determination of deoxyschizandrin in compound wuweizi capsules by RP-HPLC. Methods Deoxyschizandrin could be separated through KromasilC is （ 250 mm × 4. 6 mm,5 μm） col- umn with methanol-0. 1% acetic acid solution （82-18）by volume as a mobile phase. The flow rate was 1 ml/min and the detection wavelength was 254 nm, the temperature of column was 30℃. Results The linear range of deoxyschizandrin was 0. 1137 mg/ml - 0. 4547 mg/ml（ r = 0. 999 2, n = 6 ） and the average recovery was 99.01% with RSD 1.1% （ n = 6） for deoxyschizandrin. Conclusion The method is simple, accurate and rapid with good reproducibility, it can be used for the quality control of compound wuweizi capsules. |
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| Keywords: | HPLC Compound wuweizi capsules Deoxyschizandrin |
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