浙江畲族群体X染色体四个基因座遗传多态性

目的：了解浙江畲族群体4个X染色体短串联重复序列（short tandem repeat,X-STR）基因座DXS7132、DXS6804、DXS6799、DXS9898的等位基因及基因型频率分布。方法：从无血缘关系的260名浙江畲族个体的抗凝血中提取DNA,进行PCR扩增,聚丙烯酰胺凝胶垂直电泳（PAGE）并银染。结果：4个基因座检出6、6、6、7个等位基因,4个基因座基因型频率分布（女性个体）均符合Hardy-Weignberg平衡（P〉0.05）;观测杂合度大于0.4759,多态信息含量均大于0.4663,女性个体识别力均大于0.7096,男性个体识别力均大于0.4147。结论：4个基因座均具有较高的遗传多态性,是较理想的遗传标记系统,为浙江畲族人群法医个体识别、亲子鉴定及遗传学研究提供依据。

Genetic polymorphism of four X-short tandem repeat loci in She ethnic population in Zhejiang

Objective: To investigate the alleles and genotype frequency of DXS7132、DXS6804、DXS6799、DXS9898 short tandem repeats(STRs) loci in She ethnic population of Zhejiang.Methods: DNA were extracted respectively from anticoagulant of 260 unrelated individuals sample of She ethnic population living in Zhejiang,then were analyzed by multiplex poly-merase chain reaction(PCR) and polyacrylamide gel electrophoresis(PAGE) and silver stain detection.Results: 6、6、6、7 alleles were observed respectively in DXS7132,DXS6804,DXS6799,DXS9898 loci,and the distribution of genotype frequency(in female) match the Hardy-Weinberg equilibrium(P >0.05).The heterozygote(H) in these four loci was more than 0.4759.The polymorphism information content(PIC) were more than 0.4663.The combined power of discrimination(DP) in female and male was more than 0.7096 and 0.4147.Conclusion: The results showed that all the 4 STR loci in this study were found to have high heterozygosity and polymorphic information content,so they could provide useful markers for genetic purposes.The data obtained can be used in individual identification,paternity test and population genetics investigation of She ethnic population living in Zhejiang.