黄芪三七合剂对大鼠肾缺血再灌注损伤的作用及机制研究

目的观察黄芪三七合剂（A＆R）对肾缺血再灌注损伤（IRI）大鼠血液活性氧（ROS）变化的影响，探讨其抗IRI损伤的机制。方法雄性Sprague．Dawley（sD）大鼠30只，随机分为正常组（n=5）、假手术组（SG）（n=5）和IRl24h组（n=10），A＆R组（n=10）。造模：采用微血管夹夹闭双侧肾蒂，22vain后松开动脉夹，用5／0尼龙缝合线缝合腹部。再灌注24h后将小鼠行麻醉处死。A＆R组给予A＆R（3mL／d），假手术组及IRI24h组给予同等体积的生理盐水。采用全自动生化分析仪检测各组大鼠的肾功能，苏木精-伊红染色了解肾脏病理损害，流式细胞仪检测红细胞ROS。结果IRI24h组和A＆R组肾小管出现不同程度的管腔扩张、变性与坏死，间质炎性细胞浸润、充血水肿等变化。IRI后24h时，IRI24h组、A＆R组血清尿素氮（BUN）和肌酐（Cr）均高于假手术组、正常组，差异有统计学意义（P〈0．05）；A＆R组ROS荧光强度阳性率显著低于IRI24h组，差异有统计学意义（P〈O．05）。A＆R组肾小管损伤评分明显低IRI24h组（P〈0．05）。相关性分析发现，红细胞ROS荧光强度阳性率与’肾小管损伤评分、肌酐、尿素氮水平成正相关（r=0．917，P〈0．01；r=0．897，P〈0．01；r=0．896，P〈0．01）。结论A＆R对肾脏缺血再灌注损伤具有明显的保护作用，其机制可能为抑制血液中ROS的活性，从而抑制氧化应激对肾脏的损伤。

Research on the Effects and Mechanisms of Astragalus and Radix Notoginseng on Renal Ischemia Reperfusion Injury in Rats

Objective To investigate the effects and mechanisms of astragalus and radix notoginseng （A＆R） on the change of blood reactive oxygen species （ROS） in rats with renal isehemia reperfusion injury （IRI）. Methods A total of 32 male Sprague-Dawley rats were randomly divided into normal group （n=5）, sham group （n=5）, IRI 24-hour group （n=10） and A＆R group （n=10）. Microvaseular clips were placed to occlude both renal pedicles for 22 minutes. The abdomen was closed with 5/0 monofilament suture. Rats were euthanized 24 hours after reperfusion. A＆R group received A＆R （3 mL/d）; the sham group and IRI 24-hour group received corresponding amount of saline. Renal function was examined with automatic biochemical analyzer; renal pathological damage was detected with hematoxylin-eosin staining; and flow cytometry was used to detect the ROS of erythrocytes. Results Various degrees of luminal expansion, degeneration and necrosis, and interstitial inflammatory cell infiltration and congestion as well as edema appeared in renal tubules in IRI 24-hour and A＆R groups. Twenty-four hours after IRI, serum urea nitrogen （BUN） and creatinine （Cr） levels of IRI 24-hour group and A＆R group were significantly higher than the sham group and the normal group （P 〈 0.05）; ROS fluorescence intensity positive rate of A＆R group was significantly lower than that of the 1RI 24-hour group （P 〈 0.05）; The score of renal tubular injury of A＆R group was significantly lower than that of the IRI 24-hour group （P 〈 0.05）. Correlation analysis found that the positive rate of erythrocyte ROS fluorescence intensity was positively correlated with tubular injury score, creatinine and BUN level （r=0.917, P 〈 0.01; r=0.897, P 〈 0.01; r=0.896, P 〈 0.01）. Conclusion A＆R has a significant protective effect on renal ischemia reperfusion injury, the mechanism of which may inhibit the activation of blood ROS, thus inhibiting the damage of oxidative stress to the kidneys.