| 人早孕绒毛细胞滋养层细胞培养方法的改良 |
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| 引用本文： | 左彦珍 张孔雁 李玉红 许倩 毛晓丹 谭宇思. 人早孕绒毛细胞滋养层细胞培养方法的改良[J]. 解剖学报, 2013, 44(6): 861-864. DOI: 10.3969/j.issn.0529-1356.2013.06.022 |
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| 作者姓名： | 左彦珍 张孔雁 李玉红 许倩 毛晓丹 谭宇思 |
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| 作者单位： | 1. 承德医学院病理学教研室; 2. 药理学教研室; 3. 附属医院老年病科; 4.基础研究所， 河北 承德 067000 |
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| 基金项目： | 省教育厅项目;省自然科学基金资助项目 |
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| 摘 要： | 目的 探索一种简便高纯度的早孕滋养细胞的体外培养方法。方法 采用完整早孕绒毛通过胰蛋白酶联合胶原酶消化法分离，Percoll梯度分离法提纯体外培养，应用光镜HE染色和免疫细胞化学法检测细胞纯度。 结果 CK-7表达阳性细胞数可以达到90%以上。结论 完整绒毛消化和Percoll纯化联合可在短期内获得高纯度、高产量的滋养细胞以供后期试验，方法更加简便。
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| 关 键 词： | 滋养细胞 早孕 原代细胞培养 Percoll分离 人 |
| 收稿时间： | 2013-01-14 |
Isolation and culture of first trimester human trophoblast cell |
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| ZUO Yan-zhen ZHANG Kong-yan LI Yu-hong XU Qian MAO Xiao-dan TAN Yu-si. Isolation and culture of first trimester human trophoblast cell[J]. Acta Anatomica Sinica, 2013, 44(6): 861-864. DOI: 10.3969/j.issn.0529-1356.2013.06.022 |
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| Authors: | ZUO Yan-zhen ZHANG Kong-yan LI Yu-hong XU Qian MAO Xiao-dan TAN Yu-si |
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| Affiliation: | 1. Department of Pathology, Chengde Medical College; 2. Department of Pharmacology; 3. Department of Geriatrics, Affiliated Hospital of Chengde Medical College; 4. Institute of Basic Medical, Chengde Medical College, Hebei Chengde 067000, China）; |
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| Abstract: | Objective To research a simple method for purification of first trimester human trophoblast cell in vitro.Methods Whole first trimester human chorionic tissues were isolated with trypsin/collagenase. HE staining and immunocytochemistry methods were used to detect the tyophoblast cells purity. Results Cytokeratin-7 positive cells were more than 90%. Conclusion First trimester human Trophoblast cells can be obtained through the simple method and may offer enough cells for later experiments. |
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| Keywords: | Tyophoblast cell First trimester Primary cell culture Percoll gradient Human |
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