远端同源异型盒2和叉头盒O3a在大鼠脑皮质发育过程中的分布与表达

目的 了目的 探讨远端同源异型盒2（Dlx2）和叉头盒O3a（FoxO3a）在SD大鼠脑皮质发育过程中的表达特点。 方法 应用Real-time PCR法检测Dlx2 mRNA和FoxO3a mRNA分别在受孕11d（E11）、E13、E15、E17、E19及生后1d（P1）的表达情况；应用免疫组织化学技术显示Dlx2和FoxO3a在E11、E13、E15、E17、E19及P1蛋白的表达情况。 结果 FoxO3a与Dlx2 mRNA在E11～P1均有表达，而FoxO3a mRNA表达高峰明显早于Dlx2 mRNA；Dlx2 mRNA表达在E15～E17时大幅度上升，而在此之后始终保持这一高水平的表达；Dlx2 mRNA与FoxO3a mRNA的表达在E15～P1呈现出一致的变化趋势；Dlx2基因在E19时出现mRNA水平的高表达而未见蛋白表达。 结论 Dlx2和FoxO3a基因在胚胎后期鼠脑皮质中均有表达，且其表达趋势具有一定的一致性；两基因的分布随皮质分层而改变。

Distal-less homeobox 2/ forkhead box O3a distribution and expression in the development of rat cerebral cortex

Objective To investigate the expression of distal-less homeobox 2(Dlx2) and forkhead box O3a(FoxO3a) in developing cortex of SD rats. Methods Real-time PCR and immunohistochemical staining were performed to study the changes in Dlx2/FoxO3a expression. Results Dlx2/FoxO3a mRNA were expressed in embryonic day 11(E11)-postnatal day 1(P1), while the expression peak of FoxO3a mRNA was significantly earlier than Dlx2 mRNA. The expression of Dlx2 mRNA was significantly increased at E15-E17, and this high level expression was maintained until P1. The expression of Dlx2/FoxO3a mRNA showed a consistent trend in E15-P1. At E19,Dlx2 gene showed high expression of mRNA but no protein expression. Conclusion Dlx2 and Fox03a genes are expressed in the cerebral cortex of the late embryos,and their expression trend is consistent.The distributions of the two genes are changed with the cortical stratification.