人脑伏隔核的数字解剖

目的探讨数字人脑中伏隔核的定位、参数测量及三维显示。方法运用数控铣床完成1例45岁男性标本头部原始数据的采集,断面间距0.5mm。选取包含脑组织的连续横断面图像300幅,利用Photoshop CS软件完成尾状核、壳、伏隔核的图像分割,在重建的连续冠状断面图像上按照哈佛大学医学院的颅脑图像分割方法区分伏隔核,计算伏隔核体积及相关位置信息。利用Amira 3.1.1软件实现尾状核、壳、伏隔核的三维可视化。结果伏隔核及其毗邻结构、常用伏隔核损毁靶点清晰显示。伏隔核体积左侧为972.5mm3,右侧为830.6mm3,左侧大于右侧。伏隔核质心三维坐标,左侧(-11.0,24.4,1.3),右侧(9.3,23.9,1.7)。结论伏隔核的数字解剖能够清晰显示伏隔核的形态,明确伏隔核的体积、位置及毗邻关系。

Expression of tumor stem cell markers in the tumor sphere from esophageal squamous cells carcinoma Eca109

Objectiv To observe the expression of tumor stem cell markers P75NTR,Oct-4,Sox-2,Lin28 and Nanog in the tumor sphere from esophageal squamous cells carcinoma Eca109 and identify the esophageal squamous cell cancer stem cell marker. Methods The serum-free culture method was used for generating tumor spheres: proliferation was observed in enrichment culture tumor spheres. Small tumor spheres were obtained after 5 days culture and big and round tumor spheres appeared after 14 days culture which were collected for experiments and passaged.The expression and location of P75NTR,Oct-4,Sox-2,Lin28, and Nanog were detected by immunofluorescence cytochemistry. Results The expressions of P75NTR,Oct-4 and Lin28 were positive in the center of tumor spheres and some on cytoplasm and other in nuclei of Eca109 monolayer cells. However, Oct-4 fluorescence intensity was weaker than P75NTR.The expressions of Sox-2 and Nanog were positive in cytoplasm of tumor spheres and Eca109 monolayer cells. Conclusion The cells expressing P75NTR, Oct-4, and Lin28 in the center of the tumor sphere may be esophageal cancer stem cells.