青海省妊娠高血压综合征患者血清瘦素水平及瘦素受体的基因多态性

目的 探讨瘦素受体（LEPR）Gln223Arg（rs1137101）基因多态性与青海省妊娠高血压综合征之间的相关性。 方法 选取妊娠高血压综合征（PIH）患者107例，正常妊娠组116例，用ELISA法检测血清瘦素水平。以外周静脉血DNA为模板，采用聚合酶链反应 限制性内切酶长度片段多态性（PCR-RFLP）对LEPR 基因Gln223Arg位点基因型进行分析。 结果 PIH组血清瘦素水平高于对照组（P<0.05）。PIH组基因型频率GG型、GA型和AA型分别为49.5%、43.9%和6.5%，对照组分别为63.8%、32.8%和3.4%。PIH组和对照组LEPR基因Gln223Arg 位点等位基因G和A频率分布具有差异性。PIH组A等位基因频率高于正常妊娠组（χ²=4.60，P<0.05，OR=0.62，95%CI=0.40～0.96）。 结论 LEPR基因Gln223Arg的A等位基因是PIH遗传易感基因之一，可能是PIH的潜在危险因素。

Serum leptin and polymorphism of leptin receptor gene Gln223Arg of Qinghai patients with pregnancy-induced hypertension syndrome

Objective To investigate the relationship between polymorphism of leptin receptor(LEPR) gene Gln223Arg (rs1137101) and pregnancy induced hypertension syndrome. Methods The study consisted of 107 patients of pregnancy-induced hypertension syndrome (PIH) subjects, 116 normal pregnancy subjects were studied to detect their sera level of leptin by ELISA. The LEPR Gln223Arg genotype was detected by polymerase chain reaction fragment length polymorphism (PCR-RFLP). Results The PIH group sera leptin level was higher than control group(P<0.05).The frequences of GG,GA,AA genotype of LEPR Gln223Arg in PIH group were 49.5%,43.9% and 6.5%, in control group were 63.8%,32.8% and 3.4%. There was difference in frequency distribution of LEPR genotype between PIH group and control group. The frequency of A gene in PIH group was higher than the control group（χ²=4.60，P<0.05，OR=0.62，95%CI=0.40-0.96）. Conclusion The A allele of LEPR Gln223Arg might be a genetic marker and a latent risk factor for PIH recurrence.