首页 | 本学科首页   官方微博 | 高级检索  
     

过继转移日本血吸虫感染鼠DC亚群对过敏性哮喘肺组织病理改变及CCL2表达的抑制作用
引用本文:申妍姣,刘俊燕,杨秀珍,朱云娟,李娜,路平,李路远,王世忠,吴增强,安桂珍,纪伟华,刘佩梅. 过继转移日本血吸虫感染鼠DC亚群对过敏性哮喘肺组织病理改变及CCL2表达的抑制作用[J]. 中国病原生物学杂志, 2012, 0(3): 177-180
作者姓名:申妍姣  刘俊燕  杨秀珍  朱云娟  李娜  路平  李路远  王世忠  吴增强  安桂珍  纪伟华  刘佩梅
作者单位:天津医科大学寄生虫教研室
基金项目:天津市自然科学基金项目(No.11JCZDJC19500)
摘    要:目的通过过继转移日本血吸虫感染鼠树突状细胞(DC)亚群,探讨DC亚群在抑制过敏性哮喘中的作用。方法用CD8α和CD11c磁珠分离纯化日本血吸虫感染鼠DC亚群,分别获得CD8α-CD11c+DC和CD8α+CD11c+DC。将24只BALB/c小鼠随机分为4组:A组为健康对照组,CD8α-DC/OVA组(B组)为过继转移日本血吸虫感染鼠DCCD8α-CD11c+亚群并诱发过敏性哮喘组,CD8α+DC/OVA组(C组)为过继转移日本血吸虫感染鼠DC CD8α+CD11c+亚群并诱发过敏性哮喘组,OVA组(D组)为单纯诱发过敏性哮喘组。B、C两组小鼠分别经尾静脉过继转移5×105 DC亚群,1h后B、C和D组均开始诱发哮喘。4周后处死小鼠,取左肺做病理切片和免疫组织化学染色,观察炎症变化,检测肺组织CCL2表达情况。结果与C组和D组比较,B组肺部炎症显著减轻,按照Underwood标准,A、B、C和D等4组总评分分别为0、7.67±2.34、11.17±1.47和11.75±2.22,差异有统计学意义(P<0.05)。4组小鼠肺组织CCL2平均吸光度值分别为0.0327±0.0154、0.3967±0.0250、0.5274±0.0281和0.5631±0.0282,差异有统计学意义(P<0.05)。结论日本血吸虫感染鼠CD8α-CD11c+DC亚群对过敏性哮喘小鼠肺组织病理改变及CCL2的表达有抑制作用。

关 键 词:血吸虫,日本  过敏性哮喘  树突状细胞亚群  过继转移  免疫组化

Inhibitory effect of dendritic cell subsets from mice infected with Schistosoma japonicum on pulmonary inflammation and CCL2 levels in asthma
SHEN Yan-jiao,LIU Jun-yan,YANG Xiu-zhen,ZHU Yun-juan,LI Na,LU Ping,LI Lu-yuan,WANG Shi-zhong,WU Zeng-qiang,AN Gui-zhen,JI Wei-hua,LIU Pei-mei. Inhibitory effect of dendritic cell subsets from mice infected with Schistosoma japonicum on pulmonary inflammation and CCL2 levels in asthma[J]. Journal of Pathogen Biology, 2012, 0(3): 177-180
Authors:SHEN Yan-jiao  LIU Jun-yan  YANG Xiu-zhen  ZHU Yun-juan  LI Na  LU Ping  LI Lu-yuan  WANG Shi-zhong  WU Zeng-qiang  AN Gui-zhen  JI Wei-hua  LIU Pei-mei
Affiliation:(Department of Parasitology,Tianjin Medical University,Tianjin 300070,China)
Abstract:Objective To study the inhibition of allergic asthma by adoptive transfer of dendritic cell(DC) subsets from mice infected with Schistosoma japonicum.Methods DC subsets from mice infected with S.japonicum were respectively isolated and purified using CD8α and CD11c microbeads.Twenty four BALB/c mice were randomly divided into four groups.Group A served as the control.Each mouse in Group B was injected in the tail vein with 5×105 CD8α-CD11c+ DCs from mice infected with S.japonicum while each mouse in Group C was injected in the tail vein with 5×105 CD8α+CD11c+DCs.Group D was the asthma group.One hour after adoptive transfer,mice in Groups B,C and D were sensitized and challenged with ovalbumin to induce asthma.Mice were sacrificed after four weeks and their left lung was removed for histopathological analysis and immunohistochemical staining.Inflammatory changes were observed and levels of CCL2 expression in lung tissue were detected.Results Pulmonary inflammation diminished markedly in mice in Group B compared to mice in Groups C and D.In accordance with Underwood’s standards,average total scores for pulmonary inflammation and tissue damage in Groups A,B,C,and D were 0,7.67±2.34,11.17±1.47,and 11.75±2.22,respectively(P<0.05).Immunohistochemical staining indicated that the average optical density(AOD) of CCL2-positive regions in mouse lung tissue was 0.0327±0.0154 for Group A,0.3967±0.0250 for Group B,0.5274±0.0281 for Group C,and 0.5631±0.0282 for Group D.The difference in optical density in the groups was statically significant(P<0.05).Conclusion The CD8α-CD11c+DC subset from mice infected with S.japonicum can inhibit pulmonary inflammation and CCL2 levels in OVA-induced asthma.
Keywords:Schistosoma japonicum  asthma  dendritic cell subsets  adoptive transfer  immunohistochemistry
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号