nm23和p16基因协同表达与胃癌临床生物学特性及预后的关系

目的：检测与分析nm23,p16基因在胃癌组织中的表达，并随访5年以上，探讨其与胃癌术后复发转移及生存期的关系。方法：应用免疫组织化学法检测nm23,P16蛋白在胃癌组织中的表达，并随访5年以上，结果：84例胃癌标本中nm23阳性率46．43％，p16阳性率44．05％，胃癌组织及转移淋巴结中阳性率明显低于正常组织和胃良性息肉，而且这2类基因的表达与肿瘤侵袭深度及临床分期有关。随访中发现，阴性表达者死亡率高，转移复发率高，中位生存期短（P＜0．01），结论：nm23,p16基因蛋白异常表达在胃癌的发生发展过程中起重要作用。有可能成为临床评价肿瘤生物学行为及判断预后约标志之一。

The Relationship between nm23 and p16 Gene Synergy Expression and Gastric Cancer Biological Characteristic and Prognosis

Objective: To examine and analyze the nm23, p16 gene expression in gastric cancer tissure,and follow up patients 5 years to discuss the relationship between nm23 and p16 gene synergy expression and gastric cancer biological characteristic and prognosis.Methods: nm23 and p16 protein in gastric cancer tissue and control were detected by immunohistochemistry,and the patients had been followed up for 5 years. Results: In 84 samples of gastric cancer, nm23 positive rate was 46.43%, p16 was 44.05%, the positive rate of gastric cancer tissue and metastasitic lymph node was lower than that of normal control, normal tissue near cancer or benign polyp,and these two genes were related to the depth of tumor invasion and clinical stage.The mortality and recurrence-metastasis rate was higher in these low expression group, and had a shorter median survive period. Conclusion: Abnormal expression of nm23 and p16 gene plays a important role in gastric cancer recurrence and devolepment and may be one of markers for evaluating tumor biological behavior and prognosis.