脉冲电磁场对去势大鼠腰椎局部细胞因子及骨组织学影响

目的：探讨脉冲电磁场对去势大鼠腰椎局部细胞因子及骨组织学的影响。方法：选用健康Wistar雌性大白鼠共18只，随机分为A组（双侧卵巢切除＋脉冲电磁场治疗组），B组（双侧卵巢切除组），C组（伪去势组），每组6只。A组于术后3d置脉冲电磁场治疗，每天1次，1h／次，共治疗3个月，电磁场频率为2Hz。3个月后处死所有大鼠，取大鼠的全部腰椎和股骨，腰椎匀浆后取上清液，采用放射免疫法测定肿瘤坏死因子（TNF—α）和白细胞介素-6（IL-6）含量。股骨固定，脱钙，常规石蜡切片，HE染色观察骨组织学变化。结果：B组大鼠腰椎TNF—α含量显著高于A、C组（P〈0．01）。B组IL-6含量与A、C组比较显著增高（P〈0．01）。A组同C组比较TNF-α和IL-6含量无统计学差异。骨组织学观察：B组股骨与A、C组比较骨皮质变薄，骨髓腔扩大，骨小梁分布稀疏，骨小梁粗细不均，见断裂现象。A组与C组比较无明显差别。结论：去势大鼠腰椎TNF-α和IL-6含量显著高于正常对照组，大鼠去势后股骨骨小梁明显变细，数量减少，分布稀疏。脉冲电磁场能明显降低去势大鼠腰椎TNF—α和IL-6的含量和抑制骨吸收。

Effect of pulsed electromagnetic field on the changes of cytokine in lumbar vertebrae and bone microstructure in ovariectomized rats

Objective： To study the effect of pulsed electromagnetic field （PEM） on the changes of cytokine in lumbar vertebrae and bone microstructure in ovariectomized （OVX） rats. Methods： Eighteen healthy three - month - old female Wistar rats were divided randomly into group A （ ovariectomized group ＋ PEMs）, group B （OVX） and group C （sham operated control group）. Group A was exposed PEMs for 3 months （60 mins/time, qd）. The frequency of PEM was 2 Hz. The animals were fed for 3 months. The lumbar vertebrae and femur of the experimental rats were harvested. The levels of TNF - α and IL - 6 were measured. The femur was cut and observed under microscope. Results：The TNF - α level in group B was significantly higher than that in group A and C （P 〈0.05）. The IL-6 level in group B was significantly higher than that in group A and C（P 〈0.01 ）. The femur structure in group B was looser than that in group A and C. Concluslon：PEM can decrease the TNF -α and IL -6 level of lumbar vertebrae and can increase density of the femur in ovariectomized rats.