| 滇牡丹内生真菌的分离及其抑菌活性研究 |
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| 引用本文： | 苗翠苹,余莹,陈有为,王皎,吴少华. 滇牡丹内生真菌的分离及其抑菌活性研究[J]. 中国药学杂志, 2011, 46(10): 738-740 |
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| 作者姓名： | 苗翠苹 余莹 陈有为 王皎 吴少华 |
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| 作者单位： | 云南大学云南省微生物研究所;云南中医学院中药学院; |
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| 基金项目： | 国家自然科学基金(20772105,21062027,20502021); 云南省应用基础研究基金(2007C019M); 云南省中青年学术技术带头人后备人才基金(2008PY028) |
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| 摘 要： | 目的 研究滇牡丹内生真菌的种群组成及其代谢产物的抑菌活性，获得抗菌活性较好的菌株，以寻找新型抗菌物质。方法 采用内生菌常规分离法对健康滇牡丹植株的根、茎、叶进行内生真菌的分离鉴定。以11 种指示菌，采用纸片扩散法对分离获得的内生真菌进行抑菌活性实验。结果 从滇牡丹植物中分离到81株内生真菌，经形态鉴定，分属于19个属。抑菌实验表明，有46株内生真菌对至少1种指示菌有抑制作用，占分离总菌株的56.8%。 结论 滇牡丹内生真菌中存在着丰富的抗菌天然活性物质，可为寻找新型抗菌物质提供资源。
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| 关 键 词： | 滇牡丹 内生真菌 抑菌活性 |
| 收稿时间： | 2011-11-11; |
Study on Isolation of Endophytic Fungi from Paeonia delavayi and Their Antimicrobial Activity |
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| MIAO Cui-ping,YU Ying,CHEN You-wei,WANG Jiao,,WU Shao-hua. Study on Isolation of Endophytic Fungi from Paeonia delavayi and Their Antimicrobial Activity[J]. Chinese Pharmaceutical Journal, 2011, 46(10): 738-740 |
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| Authors: | MIAO Cui-ping YU Ying CHEN You-wei WANG Jiao WU Shao-hua |
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| Affiliation: | MIAO Cui-ping1,YU Ying1,CHEN You-wei1,WANG Jiao1,2,WU Shao-hua1(1.Yunnan Institute of Microbiology,Yunnan University,Kunming 650091,China,2.School of TCM,Yunnan University of Traditional Chinese Medicine,Kunming 650500,China) |
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| Abstract: | OBJECTIVE To isolate endophytic fungi from Paeonia delavayi and to screen the antimicrobial activities of the secondary metabolites.METHODS Endophytic fungi were isolated from the roots,stems and leaves of healthy Paeonia delavayi and identified by conventional method.The antimicrobial activities of endophytic fungi were screened against 11 microbes by paper disc diffusion method.RESULTS Eighty-one strains of endophytic fungi were isolated from Paeonia delavayi.They were identified to belong to 19 genera.An... |
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| Keywords: | Paeonia delavayi endophytic fungi antimicrobial activity |
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