高效毛细管电泳/前沿分析及其在手性药性药物-蛋白结合研究中的应用

目的：综述了高效毛细管电泳／前沿分析（HPCE／FA）方法的原理、特点、及其在手性药物－蛋白结合研究中的应用。方法：采用HPCE／FA方法。结果：不同的手性药物对映体与蛋白结合可能存在判别，同一药物对映体之间与不同蛋白结合率可能存在差别，HPCE／FA仅需极少量样品即可同时测定手性药物各对映体在蛋白结合中的游离浓度。结论：HPCE／FA是一种高效、分析迅速、样本用量极少的研究蛋白结合方法，特别是对于手性药物与昂贵不易获得的蛋白结合研究。

High-performance capillary electrophoresis /frontal analysis and its Application to the protein binding study of chiral drugs

OBJECTIVE:In this paper, the principle, feature and its application to the protein binding study of chiral drugs by High performance capillary electrophoresis /frontal analysis (HPCE/FA) are reviewed. METHODS: HPCE/FA methods are employed. RESULTS: Chiral drugs protein binding may be different between enantiomers, and the binding rate of the same drug between enantiomers with different proteins may be different too. CONCLUSION: HPCE/FA is a fast and high efficient analytical method which determine unbound chiral drug concentrations enantioselectively under drug protein binding equilibrium by ultramicro injection volume, and hence is useful especially for the binding study of proteins which are expensive and difficult to obtain.