转染P53基因对胶质瘤细胞株SHG44裸鼠致癌性的作用研究

 目的:探讨转染野生型P⁵³(wt-P⁵³)和突变型P⁵³(mtP⁵³)基因,对人胶质瘤细胞株SHG44裸鼠致瘤性的影响及意义.方法:采用质脂体介导法,分别将wt-P⁵³和mt-P⁵³基因导入人胶质瘤细胞株SHG44,体内外检测转导细胞的生长状况和裸鼠致瘤性.结果:转染mt-P⁵³基因的细胞株,G418筛选细胞集落数、软琼脂平皿细胞集落数以及裸鼠瘤组织重量和体积,均显著高于对照组(P <0.01);而转染Wt-P⁵³ 基因的细胞株均显著低于对照组(P<0.01),表明导入wt-P⁵³基因的细胞株,瘤细胞生长速度明显低于对照细胞株和导入.mt-P53基因的细胞株,即导入mt-P⁵³基因的细胞株瘤细胞生长速度最快,而导入Wt-P⁵³基因的细胞株瘤细胞生长速度最慢.结论:Wt-P⁵³基因能有效地抑制人胶质瘤细胞生长;mt-P⁵³基因则可以明显地促进瘤细胞生长.

Study on the role of P53 gene transfer on human glioma cell growth in nude mice

Objective To explore the significance and role of P⁵³gene transfer on human glioma cell growth in nude mice.Method Wild-type P⁵³ gene(wt-P⁵³)and mutant P⁵³ gene(mt-P⁵³)were transfected into the human glioma cell line SHG44.And the growth of gene-transfected cell lines were observed in vitro and in vivo.Results The tumor resulting from the cells transfected with the wt-P⁵³ gene more slowly and was smaller than that from control SHG44 cells.In contrast,the tumor from the ce-lls transfected with the mt-P⁵³ gene grew faster than that produced by cells transfected with the Wt-P⁵³ gene and that produced by control SHG44 cells.Conclusion The wild-type P⁵³ gene could inhibit the glioma cell growth in nude mice and the mutant P⁵³ gene could enhance the glioma cell growth in nude face.