三氧化二砷诱导人肝癌细胞株SMMC-7721凋亡的实验研究

 MTT法、AO/EB荧光染色法和流式细胞仪分析法观察As₂O₃对人肝癌细胞林SMMC-7721的作用.结果发现:As₂O₃可显著抑制SMMC-7721细胞的生长;经药物作用后,AO/EB染色时,肝癌细胞在显微镜下呈现典型的凋亡形态学改变;在流式细胞仪上可见亚G_l峰;凋亡呈剂量,时间依赖性特点;As₂O₃使细胞周期阻止于G₂/M期.以上表明:As₂O₃可诱导人肝癌细胞株凋亡,可望为临床应用砷剂治疗肝癌提供实验依据.

A Preliminary study on arsenic trioxide as an inducer of apoptosis in human hepatocellular carcinoma cell line

The effect of arsenic trioxide(As2O3) on human hepatocellular carcinoma (HCC) cell line SMMC-7721 was studied by using MTT, acridine orange (AO)/ethidiumbromide (EB) flurescent stainning and flow cytometry assays. The results showed that As_2O_3could significantly inhibit the growth of HCC cells in vitro. After treatment with As_2O_3, the cell's morphologic changes were consistent with the characteristics of apoptosis under nurescence microscopy and the sub-G1 peaks were observed by flow cytometry assays. It suggested that As2O3 can induce human HCC cell line SMMC7721 to undelgo apoptosis, and so As_2O_3 may haVe a potential value in clinically treating HCC.