原发性中枢神经系统淋巴瘤的临床病理、免疫表型及其与EB病毒相关性研究

目的：观察原发性中枢神经系统淋巴瘤（PCNSL）的临床病理、免疫表型及其与EB病毒的关系。方法：搜集25例PCNSL的临床资料并随访，应用单克隆抗体UCHL－1、L26、k、λ胶质纤维酸性蛋白（GFAP）和CS1－4行免疫组织化学染色，EB病毒寡核苷酸探针（EBER1／2）原位杂交，研究其免疫表型和EB病毒感染情况。结果：25例PCNSL均为B细胞淋巴瘤，EBER1／2原位杂交25例中仅2例（8％）出现阳性。结论：本组PCNSL均为B细胞激性。且与EB病毒呈低相关性。

Investigation of the clinicopathologic features, immunophenotype and Epstein-Barr virus infection of primary central nervous system lymphoma

OBJECTIVE: To investigate the clinicopathological features, immunophenotypes and EB virus infection of 25 cases of primary central nervous system lymphoma (PCNSL). METHODS: Immunohistochemical staining was performed using monoclonal antibodies, L26 (CD20), kappa,lambda for B lymphocytes, UCHL-1 (CD45RO) for T lymphocytes, CS1-4 for latent membrane protein-1 (LMP-1) and GFAP for gliocytes. In situ hybridization was used for detection of EB virus encoded small nuclear RNAs (EBER1/2). RESULTS: All of the 25 PCNSL were of B lymphocyte origin with high-grade malignancy. Only 2/25 (8%) cases showed positive signal in EBER1/2 in situ hybridization. CONCLUSION: This group of PCNSL were all B-cell lymphomas, and the role of EB virus in this group of PCNSL was uncertain.