植入前小鼠胚胎活检的实验研究

目的　以小鼠胚胎进行植入前胚胎活检的研究。　方法　通过输卵管冲洗 ,获得 4～ 8细胞期胚胎 ,用“一”字机械法和化学法分别对 4、8细胞的胚胎进行活检后继续培养 ,比较其囊胚形成及孵出情况。　结果　“一”字机械法胚胎活检后胚胎的囊胚形成率 (4细胞期 78 8% ,n =6 6 ;8细胞 99 0 % ,n =10 0 )、囊胚孵出率 (4细胞期 84 6 % ,n =5 2 ;8细胞期 94 9% ,n =99) ;化学法胚胎活检后胚胎的囊胚形成率 (4细胞期 74 1% ,n =5 8;8细胞期 92 9% ,n =12 7)、囊胚孵出率 (4细胞期88 4 % ,n =4 3;8细胞期 95 8% ,n =118)。两法均与未活检对照组比较无显著性差异 (P >0 .0 5 ) ,且两者的囊胚形成率、囊胚孵出率均无显著性差异 (P >0 .0 5 )。Logistic回归分析表明 ,细胞数越多越有利于胚胎进一步发育 ;而是否活检与活检方法对胚胎发育潜能无显著影响。　结论　用“一”字机械法在小鼠胚胎 4或 8细胞期进行单个卵裂球活检 ,不影响胚胎的发育 ,是一种较好的胚胎活检方法。

An experimental study of murine embryonic biopsy in preimplantation

Objective: To investigate the methods of murine embryonic biopsy in preimplantation Methods:Single blastomere from pre embryo at 4 or 8 cell stage was sampled by mechanical and chemical biopsy. The further development of these biopsied embryos was observed. Results: There was no significant difference in the blastocyst rate (4 cell 78.8%∶85.5%;8 cell 99 0%∶95.0%) and in the hatching rate (4 cell 84.6%∶87.8%;8 cell 94.9%∶0.8%) between the mechanical biopsied group and control. There was also no significant difference in the blastocyst rate (4 cell 74.1%∶83.8%;8 cell 92.9%∶92.8%) and in the hatching rate (4 cell 88.4%∶86 6%;8 cell 95 8%∶90.7%) between the chemical biopsied group and control. No significant difference was found in the blastocyst rate and in the hatching rate of the biopsied embryos between mechanical biopsied group and chemical biopsied group. Conclusion: Mechanical and chemical microbiopsy of pre embryo at 4 or 8 cell stage did not affect their further development.