酶联免疫法与胶体金法在乙肝表面抗原检测中的应用

目的探讨酶联免疫法与胶体金法在乙肝表面抗原检测中的应用价值。方法选取2018年1～7月我院健康体检人员血液标本780份,所有标本均采用真空管采集。运用酶联免疫法与胶体金法检测所有血液标本,统计分析酶联免疫法与胶体金法在乙肝表面抗原检测中的阳性情况,并对比酶联免疫法与胶体金法在乙肝表面抗原检测中的应用价值。结果酶联免疫法与胶体金法在乙肝表面抗原检测中的阳性率9.23%(72/780)、8.72%(68/780)之间差异不显著(P0.05)。将常规法设定为酶联免疫法,胶体金法在乙肝表面抗原检测中的敏感度为72.22%(52/72),特异度为97.74%(692/708),准确度为95.38%(744/780),阳性预测值为76.47%(52/68),阴性预测值为97.19%(692/712)。结论酶联免疫法与胶体金法在乙肝表面抗原检测中的应用价值相当,临床应该依据实际情况合理选择检测方法。

Application of enzyme-linked immunosorbent assay and colloidal gold method in detection of hepatitis B surface antigen

Objective To investigate the application value of enzyme-linked immunosorbent assay and colloidal gold assay in the detection of hepatitis B surface antigen.Methods 780 blood samples were taken from health checkups from January to July 2018 in our hospital.All specimens were collected by vacuum tube.All blood samples were detected by enzyme-linked immunosorbent assay and colloidal gold method.The positive results of enzyme-linked immunosorbent assay and colloidal gold assay in hepatitis B surface antigen detection were statistically analyzed.The application value between enzyme-linked immunosorbent assay and colloidal gold assay in the detection of hepatitis B surface antigen was compared.Results The positive rate of enzyme-linked immunosorbent assay and colloidal gold method in the detection of hepatitis B surface antigen was 9.23%(72/780)and 8.72%(68/780).The difference was not significant(P>0.05).The conventional method was set as the enzyme-linked immunosorbent assay.The sensitivity of the colloidal gold method in the detection of hepatitis B surface antigen was 72.22%(52/72),the specificity was 97.74%(692/708),and the accuracy was 95.38%(744/780),the positive predictive value was 76.47%(52/68),and the negative predictive value was 97.19%(692/712).Conclusion The application value of enzyme-linked immunosorbent assay and colloidal gold method in the detection of hepatitis B surface antigen is equivalent.The clinical method should be based on the actual situation.