| 火棘不同药用部位中槲皮素含量的比较 |
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| 引用本文： | 甘秀海,赵杨,周欣,赵超,梁志远. 火棘不同药用部位中槲皮素含量的比较[J]. 中国实验方剂学杂志, 2012, 18(11): 100-102 |
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| 作者姓名： | 甘秀海 赵杨 周欣 赵超 梁志远 |
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| 作者单位： | 1. 贵州师范学院化学与生命科学学院,贵阳,550018
2. 贵州师范大学天然药物质量控制研究中心,贵阳,550001 |
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| 基金项目： | 贵州省科技创新人才团队建设项目[黔科合人才团队(2011)4008] |
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| 摘 要： | 目的:建立反相高效液相色谱法测定火棘中槲皮素含量的方法,并比较火棘不同部位中槲皮素的含量.方法:采用90％甲醇-25％盐酸(4∶1)为溶剂提取样品,利用反相高效液相色谱法测定火棘不同部位样品中槲皮素的含量.色谱柱Hypersil ODS(4.6 mm×250 mm,5μm),流动相甲醇-0.1％磷酸水溶液(40∶ 60),流速1.0 mL· min-1,检测波长360nm,柱温25℃.结果:槲皮素在0.048 5 ～ 1.552 0μg线性关系良好,平均回收率为96.18％.结论:火棘不同部位中槲皮素的含量差异较大.
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| 关 键 词： | 火棘 反相高效液相色谱 黄酮类化合物 含量测定 |
| 收稿时间： | 2011-12-05 |
Comparison of the Contents of Quercitin in Various Medicinal Parts of Pyracantha fortuneana |
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| GAN Xiu-hai,ZHAO Yang,ZHOU Xin,ZHAO Chao and LIANG Zhi-yuan. Comparison of the Contents of Quercitin in Various Medicinal Parts of Pyracantha fortuneana[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(11): 100-102 |
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| Authors: | GAN Xiu-hai ZHAO Yang ZHOU Xin ZHAO Chao LIANG Zhi-yuan |
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| Affiliation: | The School of Chemistry and Life Science, Guizhou Normal College, Guiyang 550018, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang 550001, China;The School of Chemistry and Life Science, Guizhou Normal College, Guiyang 550018, China |
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| Abstract: | Objective:To established an RP-HPLC method for determination of quercetin in Pyracantha fortuneana and to compare quercetin contents in different medicinal parts.Method: 90%methanol-25% hydrochloride aqeous solution(4∶1) was used to extract quercetin and Hypersil ODS(4.6 mm×250 mm,5 μm) column was used to analyze quercetin content with methanol-0.1% phosphoric acid aqueous solution as mobile phases.Flow rate was 1.0 mL·min-1.Detection wavelength was set at 360 nm.Result: The standard curve of quercetin was linear in the range of 0.048 5-1.552 0 μg.The average recovery of quercetin was 98.44%.Conclusion: Quercetin contents in different medicinal parts of P.fortuneana was different. |
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| Keywords: | Pyracantha fortuneana RP-HPLC quercetin content detemination |
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