参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤的保护作用

目的:观察参芎化瘀胶囊对脑缺血再灌注大鼠脑梗死体积、脑组织水含量及形态结构改变的影响,探讨参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤的保护作用.方法:实验大鼠随机分为5组:假手术组、缺血再灌注组(模型组)、参芎化瘀胶囊高、中、低剂量治疗组( 480,240,120 mg·kg-1).ig给药7d,每天1次,第7天ig2 h后线栓法制作大脑中动脉阻断(MCAO)再灌注模型.采用TTC染色法观测脑梗死体积,干湿重法测量脑组织水含量,光镜和电镜观察脑形态结构改变情况.结果:与假手术组比较,模型组大鼠脑梗死体积增大,脑组织含水量升高,差异有显著性(均P＜0.05)；与模型组比较,参芎化瘀胶囊治疗组大鼠脑梗死体积、脑组织含水量有不同程度减少,差异有显著性(P ＜0.05,或P＜0.01),其中以高剂量组 效果最为显著.光镜和电镜下,参芎化瘀胶囊治疗组大鼠脑组织形态结构和超微结构损伤较模型组明显减轻,其中以高剂量组效果最为明显.结论:参芎化瘀胶囊对大鼠急性脑缺血再灌注损伤具有保护作用.

The Protective Effect of Shenxiong Huayu Capsule on Acute Cerebral Ischemia Reperfusion Injury in Rats

Objective: To observe the effect of Shenxiong Huayu capsule on infracted volume, contents of brain water and the changes of morphological and ultrastructure of acute cerebral ischemia reperfusion in ratsand investigate the protective effect of Shenxiong Huayu capsule on acute cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into five groups: sham operation group, ischemia reperfusion group (model group), Shenxiong Huayu capsule high, medium and low dose group(480,240,120 mg·kg^-1). Intragastric administration lasted 7 day, once a day. Middle cerebral artery occlusion (MCAO) and reperfushion model was made by an intraluminal filament method. The infracted volume was measured by TTC staining, contents of brain water was measured by dry-wet method and the morphological changes were observed using light and election microscope. Result: Compared with the sham operation group, the infracted volume and contents of brain water were increased in model group and showed a significant difference(all P<0.05); compared with the model group, the infracted volume and contents of brain water were reduced in Shenxiong Huayu capsule dose groups, showed a significant difference(P<0.05, or P<0.01),the high dose group showed most significant actions. Under the light and election microscope, the changes of morphological and ultrastructure of changes were reduced in Shenxiong Huayu capsule groups, and the high dose group showed most significant actions. Conclusion: Shenxiong Huayu capsule may relive acute cerebral ischemia reperfusion injury in rats.