野菊不同部位绿原酸和3,5-二咖啡酰奎尼酸的含量测定

目的:检测湖南衡阳产野菊不同部位(叶、花、花蕾、嫩茎及老茎)中绿原酸和3,5-二咖啡酰奎尼酸的含量.方法:以80％的乙醇为溶剂,用超声法提取野菊不同部位中的绿原酸和3,5-二咖啡酰奎尼酸.采用高效液相色谱法检测绿原酸和3,5-二咖啡酰奎尼酸的含量,以0.05％磷酸水溶液-乙腈为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长327 nm.结果:绿原酸和3,5-二咖啡酰奎尼酸的质量浓度在10～100 mg·L-1(r =0.996,r =0.992)与蜂面积呈良好的线性关系(n=5),平均回收率(n=6)分别为98.5％,99.7％.野菊叶、花、花蕾、嫩茎及老茎中绿原酸的含量分别为1.08％,0.44％,0.39％,0.58％及0.52％；3,5-二咖啡酰奎尼酸的含量分别为1.66％,1.01％,0.82％,0.93％,0.64％,其中野菊叶中绿原酸和3,5-二咖啡酰奎尼酸的含量最高.结论:野菊不同部位均含有绿原酸和3,5-二咖啡酰奎尼酸,但它们的含量存在明显差异.

Comparison Analysis of Chlorogenic Acid and 3,5-O-dicaffeoylquinic Acid in Different Parts of Chrysanthemum indicum

Objective: To analyze the contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of Chrysanthemum indicum, which sources from hengyang, Hunan province. Method: Chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of C. indicum were extracted by the method of ultrasonic method with 80% ethanol and analyzed by RP-HPLC at the wavelength of 327 nm with the flow rate of 1.0 mL·min^-1. Meanwhile, 0.05% H₃PO₄ aqueous solution and acetonitrile were used as the mobile phase with gradient elution. Result: The linear range (n=5) of chlorogenic acid (r=0.996)and 3,5-O-dicaffeoylquinic acid(r=0.992) was 10-100 mg·L^-1. The average recoveries (n=6) of chlorogenic acid and 3,5-O-dicaffeoylquinic acid were 98.5% and 99.7%,respectively. The contents of chlorogenic acid in the different parts of C. indicum including leaves, flowers, buds, tender stems and old stems was 1.08%,0.44%,0.39%,0.58% and 0.52%, respectively, while the contents of 3,5-O-dicaffeoylquinic acid was 1.66%,1.01%,0.82%,0.93% and 0.64%, respectively. The contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the leaves are the highest of the all tested samples. Conclusion: Different parts of C. indicum tested all have chlorogenic acid and 3,5-O-dicaffeoylquinic acid, but the contents of which was obvious difference.