HPLC测定芪芍方有效部位中芒柄花素的含量

目的:进一步表征芪芍方有效部位中异黄酮类化合物的含量,建立HPLC法测定芪芍方有效部位中芒柄花素含量的方法.方法:采用ODSHYPERSIL色谱柱(4.6 nm ×250 mm,5μm),乙腈-2‰磷酸水梯度洗脱,柱温35℃,流速1 mL·min -1,检测波长249 nm.结果:芒柄花素的线性回归方程为Y=6×106X - 16 320(r=0.999 8),表明芒柄花素在0.036 48～0.364 8 μg,线性关系良好.平均加样回收率为98.70％,RSD 0.77％.3批芪芍方有效部位中芒柄花素的平均含量分别为0.81,0.74,0.63 mg·g-1.结论:方法简便、准确、重复性好,可以用于芪芍方有效部位中芒柄花素的质量控制.为芪芍方有效部位中异黄酮类化合物质量控制方法的建立提供支撑,同时为有效控制芪芍方有效部位质量奠定基础.

Quantitative Determination of Formononetin in the Effective Fraction of Qishao Formula by HPLC

Objective:Establishing a method of HPLC for quantitative determination of formononetin in the effective fraction of Qishao formula to further characterize the content of isoflavones in the effective fraction of Qishao formula.Method: The procedure of HPLC-PDA was performed on the chromatographic column of ODS HYPERSIL(4.6 mm×250 mm,5 μm),and the mobile phase in the gradient elution program was acetonitrile-phosphoric acid solution(0.2%),the column temperature was at 35 ℃.The flow rate was 1 mL·min-1,and the detection wavelength was at 249 nm.Result: The regression equations was as follows: Y=6×106+06X-16 320(r=0.999 8).Formononetin showed good linear relationship from 0.036 48 to 0.364 8 μg.The average recovery was 98.70%,(RSD 0.77%).The contents of formononetin in three batches of the the effective fraction of Qishao formula was 0.81,0.74,0.63 mg·g-1,respectively.Conclusion: The method is easy and accurate with higher repeatability,which can be used in the quality control of the effective fraction of Qishao formula.It will provide support to control of the isoflavones in the effective fraction of Qishao formula.At the same time to lay the foundation for controlling its quality effectively.