整体柱HPLC同时测定白葛胶囊中4种有效成分

目的:建立一种同时快速测定白葛胶囊中葛根素、大豆苷元、欧前胡素及异欧前胡素含量的方法.方法:采用C18整体柱(4.6mm × 50 mm,2μm),甲醇(A)-0.1％冰醋酸(B)为流动相,梯度洗脱,流速1.0 mL·min -1,检测波长254 nm,进样体积5μL,柱温30℃.结果:在建立的色谱条件下,4种有效成分可获得良好的分离,且各自在一定的浓度范围内线性关系良好(r ＞0.9995),方法回收率在95％ ～ 105％.结论:所建立的方法简便、快速、可靠,可用于白葛胶囊中多种成分的快速检测.

Simultaneous Determination of Four Active Components in Baige Capsules by HPLC and Monolithic Column

Objective:a method of simultaneous and fast determination the contents of puerarin,daidzein,imperatorin and isoimperatorin in Baige capsules was established.Method: the monolithic C18 column was used to perform the separation,and the mobile phase was methanol(A)-0.1% glacial acetic acid(B) with gradient elution,the flow rate was 1.0 mL·min-1,the detection wavelength was at 254 nm and the injection volume was 5 μL,the column temperature was 30 ℃.Result: these four components could be well separated under the established chromatographic condition,the linear correlation of each component was fine in certain concentration(r>0.999 5),the recovery was between 95%-105%.Conclusion: the established method was simple,rapid and reliable and could be successfully applied in the active component detection in Baige capsules.