毛菊苣与菊苣的红外光谱三级宏观指纹鉴定

目的:建立毛菊苣与菊苣的红外光谱鉴定方法。方法:运用红外光谱三级鉴定法对毛菊苣和菊苣进行了分析鉴定。结果:两种药材的红外光谱表明毛菊苣中多糖类和芳香类成分含量高于菊苣。1 800～1 300 cm-1区域的二阶导数红外光谱中,毛菊苣的1 593 cm-1峰来自芳香类化合物,菊苣的1 660 cm-1邻近峰来自蛋白质酰胺I带,毛菊苣的1 593,1 509,1 421、1 377和1 336 cm-1峰与菊苣有显著差异;1 200～700 cm-1区域的二阶导数红外光谱中,二者在717与713 cm-1附近有相似的特征峰,而毛菊苣中的1 062和957 cm-1峰与菊苣有明显差异。二维相关红外光谱中二者差异明显,在1 800～1 500 cm-1区域,毛菊苣在1 590 cm-1附近有很强的自动峰,在1 660 cm-1附近有较弱的自动峰,菊苣在1 700～1 500 cm-1区域内有非常宽的自动峰,且在1 800～1 700 cm-1区域的自动峰也强于毛菊苣。菊苣在1 120～880 cm-1波段有2个自动峰,而毛菊苣有3个自动峰。结论:红外光谱三级鉴定法可以简单、快捷、准确地对毛菊苣与菊苣进行生药鉴定。

Study on Identification of Cichorium glandulosum and Cichorium intybus by Multi-steps Infrared Macro-fingerprint Method

Objective: The establishment of infrared spectral identification for Cichorium glandulosum and C. intybus was carried out. Method: The use of infrared spectroscopy of three identification methods for C. glandulosum and C. intybus were analyzed and identified. Result: Infrared spectra showed that sugar and aromatic component content in the C. glandulosum was higher than C. intybus. Observing their secondary derivative spectra in the range of 1 800-1 300 cm^{- 1}, the strongest peak of C. glandulosum is at 1 593 cm^-1 and it is from the aromatic compounds, and the peak of C. intybus is at 1 660 cm^-1 which from the protein amide I band, the intensity of peaks at 1 593, 1 509, 1 421, 1 377, 1 336 cm^-1 of C. glandulosum is more different from C. intybus. In 1 200-700 cm^-1, there have similar peaks at the 717, 713 cm^-1, in addition, the strongest peak of C. glandulosum is at 1 063, 957 cm^-1, more different from C. intybus. The distinction of 2D IR correlation spectra is more obvious. In 1 800-1 500 cm^-1, the stronger automatic peaks of C. glandulosum present at 1 590, 1 660 cm^-1, respectively, but those of C. intybus have a very wide area auto peak in the 1 700-1 500 cm^-1, respectively. In 1 120-880 cm^-1, C. glandulosum has three automatic peaks while C. intybus has two automatic peaks. Conclusion: The infrared spectra of three identification method are simple, fast, can be used in the identification of C. glandulosum and C. intybus.