| HPLC测定芩蒌颗粒中黄芩苷的含量 |
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| 引用本文： | 汪文来,郜志宏,于智敏,赵红霞.HPLC测定芩蒌颗粒中黄芩苷的含量[J].中国实验方剂学杂志,2012,18(11):116-117. |
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| 作者姓名： | 汪文来 郜志宏 于智敏 赵红霞 |
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| 作者单位： | 1. 中国中医科学院基础理论研究所,北京,100700
2. 山西晋城煤业集团总医院中医科,山西晋城,048006 |
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| 摘 要： | 目的:用HPLC测定芩萎颗粒中黄芩苷的含量.方法:采用HPLC,以甲醇～水-磷酸(47∶53∶0.2)为流动相,色谱柱Alltech C18(4.6 mm×250 mm,5μm),柱温25℃,流速1 mL· min-1,检测波长280 nm.结果:黄芩苷在0.061 ～0.673 μg线形关系良好,平均回收率为100.7％,RSD 1.7％.结论:该工艺方法准确、简便,可用于芩蒌颗粒中黄芩苷的含量测定.
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| 关 键 词： | 芩蒌颗粒 黄芩苷 高效液相色谱法 |
| 收稿时间： | 2011/8/23 0:00:00 |
Determination of Baicalein in Qinloukeli by HPLC |
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| WANG Wen-lai,GAO Zhi-hong,YU Zhi-min and ZHAO Hong-xia.Determination of Baicalein in Qinloukeli by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(11):116-117. |
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| Authors: | WANG Wen-lai GAO Zhi-hong YU Zhi-min and ZHAO Hong-xia |
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| Institution: | Institute of Basic Theory, China Academy of Traditional Chinese Medical Sciences, Beijing 100700, China;Department of Traditional Chinese Medicine, General Hospital of Jincheng Coal, Jincheng 048006, China;Institute of Basic Theory, China Academy of Traditional Chinese Medical Sciences, Beijing 100700, China;Institute of Basic Theory, China Academy of Traditional Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective:Determination of baicalein in Qinloukeli by HPLC.Method: The separation was performed on Alltech C18(4.6 mm×250 mm,5 μm) at 25 ℃.The mobile phase was composed of methanol-water-phosphorous acid solution(47∶53∶0.2) with a flow rate of 1.0 mL·min-1.The detective wavelength is at 280 nm.Result: Hesperidin was linear within the range of 0.057 2-0.629 2 μg(r=0.999 9).The average recovery for hesperidin was 99%,and RSD was 1.23%.Conclusion: The method was selective,accurate and simple,and suitable for controlling the quality of Qinloukeli. |
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| Keywords: | Qinlou Keli baicalein HPLC |
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