| RP-HPLC同时测定茅莓中齐墩果酸和熊果酸 |
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| 引用本文： | 邹盛勤,黄浩.RP-HPLC同时测定茅莓中齐墩果酸和熊果酸[J].中国实验方剂学杂志,2012,18(11):79-81. |
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| 作者姓名： | 邹盛勤 黄浩 |
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| 作者单位： | 宜春学院化学与生物工程学院,江西省天然药物活性成分研究重点实验室,江西宜春336000 |
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| 基金项目： | 国家高技术研究发展计划项目(2002AA2Z3217) |
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| 摘 要： | 目的:建立茅莓中齐墩果酸和熊果酸的反相高效液相色谱分析方法.方法:Kromasil C18色谱柱(4.6 mm ×250mm,5μm),流动相甲醇-1.0％磷酸溶液(90∶10),流速1.0 mL· min-1,检测波长210 nm,柱温30℃.结果:齐墩果酸、熊果酸分别在0.136 8～1.368 0,0.212 8～2.218 0 μg线性关系良好,平均回收率(n=6)分别为97.5％( RSD 1.6％)和96.9％ (RSD1.4％).结论:方法准确,操作简便,数据可靠,可用于茅莓中齐墩果酸和熊果酸的含量测定.
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| 关 键 词： | 茅莓 齐墩果酸 熊果酸 反相高效液相色谱法 含量测定 |
| 收稿时间： | 2011/7/31 0:00:00 |
Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Rubus parvifolius by RP-HPLC |
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| ZOU Sheng-qin and HUANG Hao.Simultaneous Determination of Oleanolic Acid and Ursolic Acid in Rubus parvifolius by RP-HPLC[J].China Journal of Experimental Traditional Medical Formulae,2012,18(11):79-81. |
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| Authors: | ZOU Sheng-qin and HUANG Hao |
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| Institution: | College of Chemistry and Bioengineering of Yichun University, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000, China;College of Chemistry and Bioengineering of Yichun University, Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun 336000, China |
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| Abstract: | Objective:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Rubus parvifolius.Method: The separation was achieved on a Kromasil C18 column(4.6 mm×250 mm,5 μm) at 30 ℃ using methanol-phosphoric acid solution(90∶10) as the mobile phase at a flow rate of 1.0 mL·min-1,210 nm as the detection wavelength.Result: The linear ranges of oleanolic acid and ursolic acid were from 0.136 8 to 1.368 0 μg and 0.212 8 to 2.218 0 μg,respectively.The average recoveries(n=6) for oleanolic acid and ursolic acid were 97.5%(RSD 1.6%),96.9%(RSD 1.4%),respectively.Conclusion: The proposed method is accurate,simple and applicable for determination of oleanolic acid and ursolic acid in R.parvifolius. |
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| Keywords: | Rubus parvifolius oleanolic acid ursolic acid RP-HPLC content determination |
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