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| 3个品种黄精炮制前后小分子糖含量变化 | |
| 引用本文： | 曾林燕,魏征,曹玉娜,张琳琳,宋志前,刘春生,刘振丽. 3个品种黄精炮制前后小分子糖含量变化[J]. 中国实验方剂学杂志, 2012, 18(11): 69-72 |
| 作者姓名： | 曾林燕 魏征 曹玉娜 张琳琳 宋志前 刘春生 刘振丽 |
| 作者单位： | 1. 中国中医科学院中医基础理论研究所,北京,100700 2. 中国中医科学院中医基础理论研究所,北京100700;天津中医药大学中药学院,天津300193 3. 北京中医药大学药学院,北京,100102 |
| 基金项目： | 国家自然科学基金面上课题(81073050) |
| 摘 要： | 目的:研究多花黄精、黄精和滇黄精炮制前后小分子糖组成及含量变化.方法:3个品种黄精采用酒蒸制不同时间；HPLC-ELSD法检测小分子糖种类及含量,色谱柱为BIO-RAD Aminex HPX-87C(7.8 mm×300 mm,9 μm),流动相水,流速0.4 mL·min-1,柱温78℃,蒸发光散射检测器温度为105℃,载气流量2.5 mL·min-1.结果:3个品种黄精生品中检测到的小分子糖都为蔗糖和果糖,酒蒸8h或16 h后,分别又检测到葡萄糖；3种糖含量随炮制时间的延长丽增加,然后在不同时间点又呈降低趋势；2种还原糖葡萄糖和果糖之和、以及小分子精总量都在炮制16 h达到最高,为生品的4～27倍.结论:3个品种黄精中小分子糖的组成和含量随炮制时间发生变化. |
| 关 键 词： | 黄精 高效液相色谱法 炮制 小分子糖 含量 |
| 收稿时间： | 2012-12-06 |
Content Variations of Low Molecular Weight Saccharide from Rhizoma Polygonation during Processing |
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| ZENG Lin-yan,WEI Zheng,CAO Yu-n,ZHANG Lin-lin,SONG Zhi-qian,LIU Chun-sheng and LIU Zhen-li. Content Variations of Low Molecular Weight Saccharide from Rhizoma Polygonation during Processing[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(11): 69-72 | |
| Authors: | ZENG Lin-yan WEI Zheng CAO Yu-n ZHANG Lin-lin SONG Zhi-qian LIU Chun-sheng LIU Zhen-li |
| Affiliation: | The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China;School of Chinese Pharmacy Beijing University of Chinese Medicine, Beijing 100102, China;The Institute of Chinese Medicine Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China |
| Abstract: | Objective:To study the changes of low molecular weight saccharide in Rhizoma Polygonation during processing.Method:HPLC analysis was performed on a BIO-RAD Aminex HPX-87C column(7.8 mm×300 mm,9 μm),and the mobile phase was water.The flow rate was 0.4 mL·min-1 and the temperature of the ELSD was 105 ℃.Result:Sucrose and fructose were detected in the three species of Rhizoma Polygonation raw products but not glucose,and which was detected after processed to 8 h or 16 h on the species;the total contents of reducing sugar and low molecular weight saccharide were the highest when processed to 16 h which were 4-27 times higher than those of the raw products.Conclusion:The composition and the contents of low molecular weight saccharide in three species of Rhizoma Polygonation varied during processing. |
| Keywords: | Rhizoma Polygonation HPLC processing low molecular weight saccharide content |
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